膳食辣椒素对人类衰老过程中红细胞氧化还原状态的影响。
Influence of Dietary Capsaicin on Redox Status in Red Blood Cells During Human Aging.
作者信息
Kumar Prabhanshu, Chand Subhash, Chandra Pranjal, Maurya Pawan Kumar
机构信息
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201301, India.
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, India.
出版信息
Adv Pharm Bull. 2015 Nov;5(4):583-6. doi: 10.15171/apb.2015.078. Epub 2015 Nov 30.
PURPOSE
Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is a major pungent compound found in hot peppers of the plant genus Capsicum. In vitro effects of dietary capsaicin on redox status in red blood cells during human aging have been explored.
METHODS
Total antioxidant potential of capsaicin was evaluated using Ferric reducing ability of plasma (FRAP) assay. GSH was measured as per standard protocol. The in vitro effect of capsaicin was evaluated by incubation of the cells in the assay medium with 10(-5)M capsaicin (final concentration) for 60 min at 37°C.
RESULTS
Treatment with capsaicin (10(-5)M) caused a significant (p < 0.01) increase in GSH level in all age groups. Reduced glutathione (GSH) / Oxidized glutathione (GSSG) ratio measures the redox status of the red blood cell. Significant increase in GSH level due to capsaicin, shift the GSH/GSSG ratio, thus alters the redox status of the cell.
CONCLUSION
The results conclusively prove the efficacy of the antioxidant property of capsaicin and its role in modulating the redox status of red blood cells. This evidence suggests that dietary factors that act as antioxidants to increase GSH level may contribute to a protective effect against age related diseases. This antioxidant effect may, in part, explain the high consumption of capsicum in certain regions of the world.
目的
辣椒素（8-甲基-N-香草基-6-壬酰胺）是辣椒属植物辣椒中发现的一种主要辛辣化合物。已探讨了膳食辣椒素对人体衰老过程中红细胞氧化还原状态的体外影响。
方法
使用血浆铁还原能力（FRAP）测定法评估辣椒素的总抗氧化潜力。按照标准方案测量谷胱甘肽（GSH）。通过将细胞在含有10⁻⁵M辣椒素（终浓度）的测定培养基中于37°C孵育60分钟来评估辣椒素的体外作用。
结果
用辣椒素（10⁻⁵M）处理导致所有年龄组的谷胱甘肽水平显著（p < 0.01）升高。还原型谷胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）比值衡量红细胞的氧化还原状态。辣椒素导致的谷胱甘肽水平显著升高，改变了GSH/GSSG比值，从而改变了细胞的氧化还原状态。
结论
结果确凿地证明了辣椒素抗氧化特性的功效及其在调节红细胞氧化还原状态中的作用。这一证据表明，作为抗氧化剂以增加谷胱甘肽水平的膳食因素可能有助于对与年龄相关疾病的保护作用。这种抗氧化作用可能部分解释了世界某些地区辣椒的高消费量。
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