Kumar Prabhanshu, Wadhwa Ridhima, Gupta Riya, Chandra Pranjal, Maurya Pawan Kumar
1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201303 India.
2Department of Bioscience and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781 039 India.
3 Biotech. 2018 Dec;8(12):498. doi: 10.1007/s13205-018-1524-4. Epub 2018 Nov 26.
The present study was carried out to detect intracellular quercetin uptake by RBCs during human aging. The study was carried out on 95 normal healthy subjects of both the sexes. Intracellular quercetin uptake was estimated by performing ethyl acetate extraction. A significant ( < 0.001) decline in intracellular quercetin uptake by human RBCs was observed in elderly as compared to young population, while plasma membrane redox system (PMRS) activity was significantly decreasing as a function of human age. To the best of our knowledge, we are the first to present quercetin uptake by erythrocytes during aging in humans with this study. It is hypothesized that intracellular uptake of quercetin may serve as an intracellular electron donor for plasma membrane redox system in red blood cells during cellular aging which plays an important role in extracellular dehydroascorbate reduction and ascorbate recycling.
本研究旨在检测人类衰老过程中红细胞对槲皮素的细胞内摄取情况。该研究对95名男女正常健康受试者进行。通过乙酸乙酯萃取法估算细胞内槲皮素摄取量。与年轻人群相比，老年人红细胞内槲皮素摄取量显著下降（<0.001），而质膜氧化还原系统（PMRS）活性随人类年龄增长而显著降低。据我们所知，本研究首次呈现了人类衰老过程中红细胞对槲皮素的摄取情况。据推测，在细胞衰老过程中，槲皮素的细胞内摄取可能作为红细胞质膜氧化还原系统的细胞内电子供体，这在细胞外脱氢抗坏血酸还原和抗坏血酸循环中起重要作用。
