Xie Dong, Sun Yan, Wang Lingzhen, Li Xiaoling, Zang Chuannong, Zhi Yunlai, Sun Lirong
Department of Pediatric Hematology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Department of Pediatrics, People's Hospital of Linyi, Linyi, Shandong 276000, P.R. China.
Mol Med Rep. 2016 Mar;13(3):2506-10. doi: 10.3892/mmr.2016.4812. Epub 2016 Jan 27.
Ultraviolet (UV) radiation is considered to be a potent cell-damaging agent in various cell lineages; however, the effect of UV light‑emitting diode (LED) irradiation on human cells remains unclear. The aim of the present study was to examine the effect of UV LED irradiation emitting at 280 nm on cultured HL‑60 human leukemia cells, and to explore the underlying mechanisms. HL‑60 cells were irradiated with UV LED (8, 15, 30 and 60 J/m2) and incubated for 2 h after irradiation. The rates of cell proliferation and apoptosis, the cell cycle profiles and the mRNA expression of B‑cell lymphoma 2 (Bcl‑2) were detected using cell counting kit‑8, multicaspase assays, propidium iodide staining and reverse transcription‑quantitative polymerase chain reaction, respectively. The results showed that UV LED irradiation (8‑60 J/m2) inhibited the proliferation of HL‑60 cells in a dose‑dependent manner. UV LED at 8‑30 J/m2 induced dose‑dependent apoptosis and G0/G1 cell cycle arrest, and inhibited the expression of Bcl‑2 mRNA, while UV LED at 60 J/m2 induced necrosis. In conclusion, 280 nm UV LED irradiation inhibits proliferation and induces apoptosis and necrosis in cultured HL‑60 cells. In addition, the cell cycle arrest at the G0/G1 phase and the downregulation of Bcl‑2 mRNA expression were shown to be involved in UV LED-induced apoptosis.
紫外线（UV）辐射被认为是一种对各种细胞谱系具有强大细胞损伤作用的因子；然而，发光二极管（LED）紫外线照射对人类细胞的影响仍不清楚。本研究的目的是检测280 nm发射波长的UV LED照射对培养的HL-60人白血病细胞的影响，并探讨其潜在机制。用UV LED（8、15、30和60 J/m²）照射HL-60细胞，并在照射后孵育2小时。分别使用细胞计数试剂盒-8、多caspase检测、碘化丙啶染色和逆转录-定量聚合酶链反应检测细胞增殖和凋亡率、细胞周期分布以及B细胞淋巴瘤2（Bcl-2）的mRNA表达。结果表明，UV LED照射（8-60 J/m²）以剂量依赖性方式抑制HL-60细胞的增殖。8-30 J/m²的UV LED诱导剂量依赖性凋亡和G0/G1细胞周期阻滞，并抑制Bcl-2 mRNA的表达，而60 J/m²的UV LED诱导坏死。总之，280 nm UV LED照射抑制培养的HL-60细胞的增殖并诱导凋亡和坏死。此外，G0/G1期细胞周期阻滞和Bcl-2 mRNA表达下调被证明与UV LED诱导的凋亡有关。
