Cuvas Apan Ozgun, Ozer Murat Atabey, Takir Selcuk, Apan Alparslan, Sengul Demet
Department of Anesthesiology and Intensive Care Medicine, Giresun University Medical Faculty, Nizamiye Yerleskesi, Orhan Yılmaz Caddesi, Mumcular Sokak No:1, Merkez, 28200, Giresun, Turkey.
Department of Ophthalmology, Giresun University Medical Faculty, Giresun, Turkey.
Int Ophthalmol. 2016 Oct;36(5):675-80. doi: 10.1007/s10792-016-0184-9. Epub 2016 Jan 28.
In this study, we aimed to investigate the effects of topical tramadol administration on corneal wound healing, and examine ophthalmic structures and intraocular pressure 7 days after tramadol administration. The experiments were conducted on eight male Wistar rats (250-300 g). After ophthalmic examination, epithelial cell layers in the central cornea were wounded. Rats received 30 μL of tramadol hydrochloride in one eye (Group Tramadol) and the same volume of vehicle in the other (Group Control) every 12 h for 7 days. Both eyes were stained with fluorescein dye, photographed, and wound area was calculated every 8 h until complete healing was observed. Eye blink frequency and corneal reflex tests were measured before and after drug administrations. After 7 days, slit lamp biomicroscopy, fundoscopy, Goldmann applanation tonometry, and histological evaluation were performed. There was no difference in the corneal wound healing rates between the tramadol and control groups. Reduction in wound area over time was also similar; group-time interaction was insignificant (F = 738.911; p = 0.225). Tramadol application resulted in blinking and blepharospasm for 30 s, but vehicle did not. Corneal reflex was intact and eye blink frequency test results were similar in all measurement times in both groups. Slit lamp biomicroscopy, fundoscopy, and intraocular pressures were within normal range. Corneal cells appeared unaffected by the repeated doses of tramadol for 7 days. Topical tramadol application on the cornea did not cause any side effect, except for initial temporary blinking and blepharospasm. Corneal wound healing was not affected, either.
在本研究中，我们旨在调查局部应用曲马多对角膜伤口愈合的影响，并在应用曲马多7天后检查眼部结构和眼压。实验在八只雄性Wistar大鼠（250 - 300克）身上进行。眼科检查后，对中央角膜的上皮细胞层造成损伤。大鼠每12小时在一只眼睛中接受30μL盐酸曲马多（曲马多组），另一只眼睛接受相同体积的赋形剂（对照组），持续7天。每8小时用荧光素染料对双眼进行染色、拍照，并计算伤口面积，直至观察到完全愈合。在给药前后测量眨眼频率和角膜反射测试。7天后，进行裂隙灯生物显微镜检查、眼底镜检查、Goldmann压平眼压测量和组织学评估。曲马多组和对照组之间的角膜伤口愈合率没有差异。伤口面积随时间的减少也相似；组间时间交互作用不显著（F = 738.911；p = 0.225）。应用曲马多会导致30秒的眨眼和眼睑痉挛，但赋形剂不会。两组在所有测量时间的角膜反射均完整，眨眼频率测试结果相似。裂隙灯生物显微镜检查、眼底镜检查和眼压均在正常范围内。连续7天重复给予曲马多后，角膜细胞似乎未受影响。除了最初的短暂眨眼和眼睑痉挛外，局部应用曲马多对角膜没有引起任何副作用。角膜伤口愈合也未受影响。
