用于将基因递送至哺乳动物细胞的包膜修饰杆状病毒的产生
Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells.
作者信息
Hofmann Christian
机构信息
Roche Pharma AG, Emil-Barell-Str. 1, 79639, Grenzach-Wyhlen, Germany.
出版信息
Methods Mol Biol. 2016;1350:491-504. doi: 10.1007/978-1-4939-3043-2_26.
Genetically modified baculoviruses can efficiently deliver and express genes in mammalian cells. The major prerequisite for the expression of a gene transferred by baculovirus is its control by a promoter that is active in mammalian cells. This chapter describes methods for producing second generation baculovirus vectors through modification of their envelope. Envelope modified baculoviruses offer additional new applications of the system, such as their use in in vivo gene delivery, targeting, and vaccination. Methods of generating a recombinant baculovirus vector with a modified envelope and its amplification and purification, including technical scale production, are discussed. A variety of notes give clues regarding specific technical procedures. Finally, methods to analyze the virus and transduction procedures are presented.
基因改造的杆状病毒能够在哺乳动物细胞中高效传递并表达基因。杆状病毒所转移基因得以表达的主要前提是该基因受在哺乳动物细胞中具有活性的启动子控制。本章介绍了通过修饰杆状病毒包膜来生产第二代杆状病毒载体的方法。包膜修饰的杆状病毒为该系统带来了更多新应用，比如其在体内基因传递、靶向和疫苗接种方面的应用。文中讨论了生成具有修饰包膜的重组杆状病毒载体及其扩增和纯化的方法，包括技术规模生产。各种注释提供了有关特定技术程序的线索。最后，介绍了分析病毒和转导程序的方法。
Zotero 插件