与乳腺癌进展相关的成像基质金属蛋白酶活性

Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.

作者信息

Fields Gregg B, Stawikowski Maciej J

机构信息

Department of Chemistry & Biochemistry, Florida Atlantic University, Jupiter, FL, 33458, USA.

Department of Chemistry, The Scripps Research Institute/Scripps Florida, Jupiter, FL, 33458, USA.

出版信息

Methods Mol Biol. 2016;1406:303-29. doi: 10.1007/978-1-4939-3444-7_25.

DOI:10.1007/978-1-4939-3444-7_25

PMID:26820965

Abstract

Proteolysis has been cited as an important contributor to cancer initiation and progression. One can take advantage of tumor-associated proteases to selectively deliver imaging agents. Protease-activated imaging systems have been developed using substrates designed for hydrolysis by members of the matrix metalloproteinase (MMP) family. We presently describe approaches by which one can optically image matrix metalloproteinase activity implicated in breast cancer progression, with consideration of selective versus broad protease probes.

摘要

蛋白水解作用被认为是癌症发生和发展的一个重要因素。人们可以利用肿瘤相关蛋白酶来选择性地递送成像剂。已经开发出了蛋白酶激活成像系统，该系统使用了为基质金属蛋白酶（MMP）家族成员水解而设计的底物。我们目前描述了一些方法，通过这些方法可以对与乳腺癌进展相关的基质金属蛋白酶活性进行光学成像，同时考虑选择性与广谱蛋白酶探针。