印度马哈拉施特拉邦狂犬病病毒核蛋白基因的分子特征分析
Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India.
作者信息
Mehta S, Charan P, Dahake R, Mukherjee S, Chowdhary A
机构信息
Department of Virology and Immunology, Haffkine Institute for Training, Research and Testing, Mumbai, Maharashtra, India.
出版信息
J Postgrad Med. 2016 Apr-Jun;62(2):105-8. doi: 10.4103/0022-3859.175006.
CONTEXT
Rabies poses a serious public health concern in developing countries such as India.
AIMS
The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India.
MATERIALS AND METHODS
Nucleoprotein gene from eight (of total 20 suspected samples) rabies cases that tested positive for rabies antigen using reverse transcriptase-polymerase chain reaction (RT-PCR) were sequenced.
RESULTS
Sequence analysis using basic local alignment search tool (BLAST) and multiple sequence alignment (MSA) and phylogenetic analysis showed similarity to previously reported sequences from India and those of Arctic lineages.
CONCLUSIONS
The circulating RABV strains in Maharashtra, India show genetic relatedness to RABV strains reported from Indo-Arctic lineages and India-South and Japan.
背景
狂犬病在印度等发展中国家构成严重的公共卫生问题。
目的
本研究聚焦于对从印度马哈拉施特拉邦接收的人类临床标本中的街狂犬病毒（RABV）进行分子诊断。
材料与方法
对20份疑似样本中的8份狂犬病病例的核蛋白基因进行测序，这些病例使用逆转录聚合酶链反应（RT-PCR）检测狂犬病抗原呈阳性。
结果
使用基本局部比对搜索工具（BLAST）进行序列分析、多序列比对（MSA）以及系统发育分析，结果显示与先前报道的来自印度的序列以及北极谱系的序列相似。
结论
印度马哈拉施特拉邦流行的RABV毒株与印度-北极谱系以及印度南部和日本报道的RABV毒株存在遗传相关性。
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