Ul'masov T N, Gulov M K, Aliev K A, Andrianov V M, Piruzian E S
Mol Gen Mikrobiol Virusol. 1989 Aug(8):17-20.
The fragments of cotton Gossipium hirsutum c.v. 108-f chloroplast genome were cloned in Escherichia coli cells. The cloned psbA and rbcL genes have been selected using the heterologous probes from spinach. The preliminary attempts to clone the complete psbA gene in pUC19 vector failed, probably, due to the toxicity of its product to Escherichia coli cells, and its 5'- and 3'-ends were cloned separately. Reconstruction experiments revealed that while the complete psbA gene was unable to be stably inherited by Escherichia coli cells, its structural part lacking the promoter region could be readily cloned in the bacterial cells.
将陆地棉（Gossipium hirsutum c.v. 108-f）叶绿体基因组片段克隆到大肠杆菌细胞中。利用菠菜的异源探针筛选出已克隆的psbA和rbcL基因。在pUC19载体中克隆完整psbA基因的初步尝试失败了，可能是由于其产物对大肠杆菌细胞有毒性，因此分别克隆了其5′端和3′端。重组实验表明，虽然完整的psbA基因不能被大肠杆菌细胞稳定遗传，但其缺少启动子区域的结构部分可以很容易地克隆到细菌细胞中。
