Hide G, Gray A, Harrison C M, Tait A
Wellcome Unit of Molecular Parasitology, Department of Veterinary Parasitology, University of Glasgow, U.K.
Mol Biochem Parasitol. 1989 Aug;36(1):51-9. doi: 10.1016/0166-6851(89)90199-0.
Considerable advances have been made in our understanding of cell growth regulation in mammalian cells. In particular, studies on transformed and normal cells have highlighted the contribution of growth factor-related control mechanisms in cell growth regulation. We set out to investigate whether host growth factors are involved in the growth regulation of the parasitic protozoan Trypanosoma brucei. We demonstrate that antibodies to the mammalian epidermal growth factor (EGF) receptor bind to the trypanosome T. brucei and, that these antibodies recognise a surface polypeptide of 135 kDa. This polypeptide is one of only two polypeptides in parasite extracts that bind EGF. Furthermore, EGF modifies protein kinase activity and growth rate of trypanosomes in vitro. These results lead to the conclusion that T. brucei has a surface growth factor receptor with considerable homology to the EGF receptor, and raise the possibility that growth factor interactions similar to those found in mammalian cells are involved in cell growth regulation in trypanosomes.
我们对哺乳动物细胞中细胞生长调控的理解取得了长足进展。特别是，对转化细胞和正常细胞的研究突出了生长因子相关控制机制在细胞生长调控中的作用。我们着手研究宿主生长因子是否参与寄生原生动物布氏锥虫的生长调控。我们证明，针对哺乳动物表皮生长因子（EGF）受体的抗体可与布氏锥虫结合，且这些抗体识别一种135 kDa的表面多肽。该多肽是寄生虫提取物中仅有的两种能结合EGF的多肽之一。此外，EGF在体外可改变锥虫的蛋白激酶活性和生长速率。这些结果得出结论，布氏锥虫具有与EGF受体具有相当同源性的表面生长因子受体，并增加了类似于在哺乳动物细胞中发现的生长因子相互作用参与锥虫细胞生长调控的可能性。
