鲍曼不动杆菌的外膜孔蛋白OmpW参与铁摄取和黏菌素结合。
The outer membrane porin OmpW of Acinetobacter baumannii is involved in iron uptake and colistin binding.
作者信息
Catel-Ferreira Manuella, Marti Sara, Guillon Laurent, Jara Luis, Coadou Gaël, Molle Virginie, Bouffartigues Emeline, Bou German, Shalk Isabelle, Jouenne Thierry, Vila-Farrés Xavier, Dé Emmanuelle
机构信息
CNRS UMR 6270 & FR3038, Normandie Univ, Laboratoire Polymères, Biopolymères & Surfaces, Université de Rouen, Mont Saint Aignan, France.
Center for International Health Research, CRESIB, Hospital Clínic, University of Barcelona, Spain.
出版信息
FEBS Lett. 2016 Jan;590(2):224-31. doi: 10.1002/1873-3468.12050. Epub 2016 Jan 11.
This study was undertaken to characterize functions of the outer membrane protein OmpW, which potentially contributes to the development of colistin- and imipenem-resistance in Acinetobacter baumannii. Reconstitution of OmpW in artificial lipid bilayers showed that it forms small channels (23 pS in 1 m KCl) and markedly interacts with iron and colistin, but not with imipenem. In vivo, (55) Fe uptake assays comparing the behaviours of ΔompW mutant and wild-type strains confirmed a role for OmpW in A. baumannii iron homeostasis. However, the loss of OmpW expression did not have an impact on A. baumannii susceptibilities to colistin or imipenem.
本研究旨在表征外膜蛋白OmpW的功能，该蛋白可能与鲍曼不动杆菌对黏菌素和亚胺培南耐药性的产生有关。将OmpW重组到人工脂质双层中显示，它形成小通道（在1 M KCl中为23 pS），并与铁和黏菌素显著相互作用，但不与亚胺培南相互作用。在体内，通过比较ΔompW突变株和野生型菌株行为的(55)Fe摄取试验证实了OmpW在鲍曼不动杆菌铁稳态中的作用。然而，OmpW表达的缺失对鲍曼不动杆菌对黏菌素或亚胺培南的敏感性没有影响。
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