Department of Science, Roma Tre University, Viale G. Marconi 446, 00146 Rome, Italy.
Norwich Medical School, University of East Anglia, Rosalind Franklin Road, Norwich Research Park, Norwich, NR4 7UQ, UK.
Microb Genom. 2023 Aug;9(8). doi: 10.1099/mgen.0.001080.
Iron is essential for growth in most bacteria due to its redox activity and its role in essential metabolic reactions; it is a cofactor for many bacterial enzymes. The bacterium is a multidrug-resistant nosocomial pathogen. responds to low iron availability imposed by the host through the exploitation of multiple iron-acquisition strategies, which are likely to deliver iron to the cell under a variety of environmental conditions, including human and animal infection. To date, six different gene clusters for active iron uptake have been described in , encoding protein systems involved in (i) ferrous iron uptake (); (ii) haem uptake ( and ); and (iii) synthesis and transport of the baumannoferrin(s) (), acinetobactin (/) and fimsbactin(s) () siderophores. Here we describe the structure, distribution and phylogeny of iron-uptake gene clusters among >1000 genotypically diverse isolates, showing that , , and / clusters are very prevalent across the dataset, whereas the additional haem-uptake system is only present in a portion of the dataset and the gene cluster is very rare. Since the expression of multiple iron-uptake clusters can be linked to virulence, the presence of the additional haem-uptake system may have contributed to the success of some clones.
铁对于大多数细菌的生长是必不可少的，因为它具有氧化还原活性和在必要代谢反应中的作用；它是许多细菌酶的辅因子。 是一种多药耐药的医院病原体。 通过利用多种铁获取策略来响应宿主施加的低铁可用性，这些策略可能在各种环境条件下将铁递送到细胞中，包括人类和动物感染。迄今为止，已经在 中描述了六个不同的活性铁摄取基因簇，编码参与（i）亚铁摄取（）；（ii）卟啉摄取（和）；和（iii）baumannoferrin(s) ()、acinetobactin (/)和 fimsbactin(s) () 铁载体的合成和运输的蛋白系统。在这里，我们描述了铁摄取基因簇在 >1000 个基因型多样的 分离株中的结构、分布和系统发育，表明 、 、 和 / 簇在整个数据集上非常普遍，而额外的卟啉摄取系统 仅存在于数据集的一部分中，而 基因簇非常罕见。由于多个铁摄取簇的表达可能与毒力有关，额外的卟啉摄取系统 的存在可能有助于一些 克隆的成功。
