Wu Jian, Seng Shanshan, Carianopol Carina, Sui Juanjuan, Yang Qiuyan, Zhang Fengqin, Jiang Huiru, He Junna, Yi Mingfang
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China.
Department of Biological Sciences, University of Toronto, Toronto, Ontario, Canada.
Biochem Biophys Res Commun. 2016 Feb 26;471(1):198-204. doi: 10.1016/j.bbrc.2016.01.146. Epub 2016 Jan 28.
Abscisic acid (ABA) is an important phytohormone controlling seed dormancy. AFPs (ABA INSENSITIVE FIVE BINDING PROTEINS) are reported to be negative regulators of the ABA signaling pathway. The involvement of AFPs in dormant vegetative organs remains poorly understood. Here, we isolated and characterized a novel AFP family member from Gladiolus dormant cormels, GhAFP-like, containing three conserved domains of the AFP family. Quantitative PCR analysis revealed that GhAFP-like was expressed in dormant organs and its expression was down-regulated along with corm storage. GhAFP-like was verified to be a nuclear-localized protein. Overexpressing GhAFP-like in Arabidopsis thaliana not only showed weaker seed dormancy with insensitivity to ABA, but also changed the expression of some ABA related genes. In addition, a primary root elongation assay showed GhAFP-like may involve in auxin signaling response. The results in this study indicate that GhAFP-like acts as a negative regulator in ABA signaling and is related to dormancy.
脱落酸（ABA）是一种控制种子休眠的重要植物激素。据报道，AFPs（脱落酸不敏感5结合蛋白）是ABA信号通路的负调控因子。AFPs在休眠营养器官中的作用仍知之甚少。在这里，我们从唐菖蒲休眠小种球中分离并鉴定了一个新的AFP家族成员GhAFP-like，它包含AFP家族的三个保守结构域。定量PCR分析表明，GhAFP-like在休眠器官中表达，其表达随着球茎储存而下调。GhAFP-like被证实是一种定位于细胞核的蛋白质。在拟南芥中过表达GhAFP-like不仅表现出较弱的种子休眠且对ABA不敏感，还改变了一些与ABA相关基因的表达。此外，主根伸长试验表明GhAFP-like可能参与生长素信号响应。本研究结果表明，GhAFP-like在ABA信号传导中起负调控作用，且与休眠相关。
