Jiang Yan, Zhu Rongjin, Luo Li, Mu Qiuju, Zhu Yanxin, Luo Hong, Zou Xijun, Shen Xiangchun
Department of Microbiology, Guizhou Medical University, Guiyang, PR China.
Intervirology. 2015;58(6):343-9. doi: 10.1159/000442588. Epub 2016 Feb 2.
To investigate the protective effect of recombinant mouse β-defensin 3 (rMBD3) against coxsackievirus B3 (CVB3)-induced myocarditis in mice.
CVB3-infected HeLa cells were treated with rMBD3, and the titer of CVB3 and the proliferative activities of the cells were determined. CBV3-infected BALB/c mice were divided into five groups: rMBD3 high (5 mg/kg/day, q.d.), rMBD3 low (2.5 mg/kg/day, q.d.), ribavirin (10 mg/kg/day, q.d.), normal control, and myocarditis control. On days 5 and 12 after treatment, 3 mice from each group were sacrificed and serum lactate dehydrogenase, creatine kinase-MB isozyme, and tumor necrosis factor-α levels were determined. The heart index and the inflammation of myocardial tissue were also assessed. On day 5, the CVB3 50% tissue culture infective dose (TCID50) values of heart tissues were measured.
rMBD3 inhibited the replication of CVB3 and protected HeLa cells from infection. rMBD3 reduced the CVB3 titer markedly and inhibited the pathological reaction of cardiac myocytes to viral myocarditis. Treatment with rMBD3 improved the cell survival rate and reduced the cardiac index.
rMBD3 was demonstrated to possess anti-CVB3 activity in vivo and in vitro.
研究重组小鼠β-防御素3（rMBD3）对柯萨奇病毒B3（CVB3）诱导的小鼠心肌炎的保护作用。
用rMBD3处理CVB3感染的HeLa细胞，测定CVB3滴度和细胞增殖活性。将感染CVB3的BALB/c小鼠分为五组：rMBD3高剂量组（5mg/kg/天，每日一次）、rMBD3低剂量组（2.5mg/kg/天，每日一次）、利巴韦林组（10mg/kg/天，每日一次）、正常对照组和心肌炎对照组。治疗后第5天和第12天，每组处死3只小鼠，测定血清乳酸脱氢酶、肌酸激酶-MB同工酶和肿瘤坏死因子-α水平。还评估了心脏指数和心肌组织炎症。在第5天，测量心脏组织的CVB3 50%组织培养感染剂量（TCID50）值。
rMBD3抑制CVB3复制，保护HeLa细胞免受感染。rMBD3显著降低CVB3滴度，抑制心肌细胞对病毒性心肌炎的病理反应。rMBD3治疗提高了细胞存活率，降低了心脏指数。
rMBD3在体内和体外均表现出抗CVB3活性。
