Santos Aldenor G, Regis Ana Carla D, da Rocha Gisele O, Bezerra Marcos de A, de Jesus Robson M, de Andrade Jailson B
Universidade Federal da Bahia, Instituto de Química, Campus de Ondina, 40170-115 Salvador, BA, Brazil; Instituto Nacional de Ciência e Tecnologia INCT, Universidade Federal da Bahia, 40170-115 Salvador, BA, Brazil; Centro Interdisciplinar em Energia e Ambiente - CIEnAm, Universidade Federal da Bahia, 40170-115 Salvador, BA, Brazil.
Instituto Nacional de Ciência e Tecnologia INCT, Universidade Federal da Bahia, 40170-115 Salvador, BA, Brazil; Centro Interdisciplinar em Energia e Ambiente - CIEnAm, Universidade Federal da Bahia, 40170-115 Salvador, BA, Brazil; Universidade Estadual do Sudoeste da Bahia, Campus de Jequié, 45210340 Jequié, BA, Brazil.
J Chromatogr A. 2016 Feb 26;1435:6-17. doi: 10.1016/j.chroma.2016.01.018. Epub 2016 Jan 13.
The method allowed simultaneous characterization of PAHs, nitro-PAHs and quinones in atmospheric particulate matter. This method employs a miniaturized micro-extraction step that uses 500 μL of an acetonitrile-dichloromethane mix and instrumental analysis by means of a high-resolution GC-MS. The method was validated using the SRM1649b NIST standard reference material as well as deuterated internal standards. The results are in good agreement with the certified values and show recoveries between 75% and 145%. Limit of detection (LOD) values for PAHs were found to be between 0.5 pg (benzo[a]pyrene) to 2.1 pg (dibenzo[a,h]anthracene), for nitro-PAHs ranged between 3.2 pg (1-nitrobenzo[e]pyrene) and 22.2 pg (3-nitrophenanthrene), and for quinones ranged between 11.5 pg (1,4-naphthoquinone) and 458 pg (9,10-phenanthraquinone). The validated method was applied to real PM10 samples collected on quartz fiber filters. Concentrations in the PM10 samples ranged from 0.06 to 15 ng m(-3) for PAHs, from<LOD to 69.4 ng m(-3) for nitro-PAHs, and from 0.27 to 115 ng m(-3) for quinones. This procedure was found to be precise, accurate and suitable to be employed for determination of polycyclic aromatic compounds in airborne particles from both polluted and non-polluted atmospheres.
该方法能够同时对大气颗粒物中的多环芳烃（PAHs）、硝基多环芳烃和醌类进行表征。此方法采用了一个小型化的微萃取步骤，该步骤使用500 μL乙腈 - 二氯甲烷混合液，并通过高分辨率气相色谱 - 质谱联用仪进行仪器分析。该方法使用SRM1649b NIST标准参考物质以及氘代内标进行了验证。结果与认证值高度吻合，回收率在75%至145%之间。多环芳烃的检测限（LOD）值在0.5 pg（苯并[a]芘）至2.1 pg（二苯并[a,h]蒽）之间，硝基多环芳烃的检测限范围在3.2 pg（1 - 硝基苯并[e]芘）至22.2 pg（3 - 硝基菲）之间，醌类的检测限范围在11.5 pg（1,4 - 萘醌）至458 pg（9,10 - 菲醌）之间。经验证的该方法被应用于在石英纤维滤膜上采集的实际PM10样品。PM10样品中多环芳烃的浓度范围为0.06至15 ng m(-3)，硝基多环芳烃的浓度范围为<LOD至69.4 ng m(-3)，醌类的浓度范围为0.27至115 ng m(-3)。该程序被证明是精确、准确的，适用于测定来自污染和未污染大气的空气中颗粒物中的多环芳烃化合物。
