Mills J W, Ferm V H
Department of Anatomy, Dartmouth Medical School, Hanover, New Hampshire 03756.
Toxicol Appl Pharmacol. 1989 Nov;101(2):245-54. doi: 10.1016/0041-008x(89)90273-1.
The effect of cadmium on F-actin and microtubules of Madin-Darby Canine Kidney cells was studied by cytochemical methods. A 6-hr exposure to cadmium (10 microns) in a buffered salt solution resulted in the breakdown of actin filaments, particularly those associated with both the stress fibers and the lateral membranes in areas of intercellular contact. Microtubules were not dramatically altered during this exposure period and cell viability, determined by trypan blue exclusion, was similar to controls. The effect of cadmium on actin was reversible if the cells were returned to culture medium. The results indicate that one possible mechanism of cadmium toxicity is via an effect on the organization of actin filaments.
采用细胞化学方法研究了镉对犬肾细胞（Madin-Darby Canine Kidney cells）中F-肌动蛋白和微管的影响。在缓冲盐溶液中暴露于镉（10微米）6小时，导致肌动蛋白丝解体，特别是那些与应力纤维以及细胞间接触区域的侧膜相关的肌动蛋白丝。在此暴露期间，微管没有明显改变，通过台盼蓝排斥法测定的细胞活力与对照组相似。如果将细胞放回培养基中，镉对肌动蛋白的影响是可逆的。结果表明，镉毒性的一种可能机制是通过对肌动蛋白丝组织的影响。
