肠炎沙门氏菌SE86产生的生物表面活性剂可增强生菜（菊科莴苣属）叶片上的附着力及对消毒剂的抗性。
Biosurfactant Produced by Salmonella Enteritidis SE86 Can Increase Adherence and Resistance to Sanitizers on Lettuce Leaves (Lactuca sativa L., cichoraceae).
作者信息
Rossi Eliandra M, Beilke Luniele, Kochhann Marília, Sarzi Diana H, Tondo Eduardo C
机构信息
Laboratório de Microbiologia, Departamento de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa CatarinaSão Miguel do Oeste, Brazil; Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do SulPorto Alegre, Brazil.
Laboratório de Microbiologia, Departamento de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina São Miguel do Oeste, Brazil.
出版信息
Front Microbiol. 2016 Jan 22;7:9. doi: 10.3389/fmicb.2016.00009. eCollection 2016.
Salmonella Enteritidis SE86 is an important foodborne pathogen in Southern Brazil and it is able to produce a biosurfactant. However, the importance of this compound for the microorganism is still unknown. This study aimed to investigate the influence of the biosurfactant produced by S. Enteritidis SE86 on adherence to slices of lettuce leaves and on resistance to sanitizers. First, lettuce leaves were inoculated with S. Enteritidis SE86 in order to determine the amount of biosurfactant produced. Subsequently, lettuce leaves were inoculated with S. Enteritidis SE86 with and without the biosurfactant, and the adherence and bacterial resistance to different sanitization methods were evaluated. S. Enteritidis SE86 produced biosurfactant after 16 h (emulsification index of 11 to 52.15 percent, P < 0.05) and showed greater adherence capability and resistance to sanitization methods when the compound was present. The scanning electron microscopy demonstrated that S. Enteritidis was able to adhere, form lumps, and invade the lettuce leaves' stomata in the presence of the biosurfactant. Results indicated that the biosurfactant produced by S. Enteritidis SE86 contributed to adherence and increased resistance to sanitizers when the microorganism was present on lettuce leaves.
肠炎沙门氏菌SE86是巴西南部一种重要的食源性病原体，它能够产生一种生物表面活性剂。然而，这种化合物对该微生物的重要性仍不清楚。本研究旨在调查肠炎沙门氏菌SE86产生的生物表面活性剂对生菜叶片切片黏附性以及对消毒剂抗性的影响。首先，用肠炎沙门氏菌SE86接种生菜叶片，以确定产生的生物表面活性剂的量。随后，分别用含有和不含有生物表面活性剂的肠炎沙门氏菌SE86接种生菜叶片，并评估其对不同消毒方法的黏附性和细菌抗性。肠炎沙门氏菌SE86在16小时后产生了生物表面活性剂（乳化指数为11%至52.15%，P<0.05），并且当存在该化合物时，表现出更强的黏附能力和对消毒方法的抗性。扫描电子显微镜显示，在生物表面活性剂存在的情况下，肠炎沙门氏菌能够黏附、形成团块并侵入生菜叶片的气孔。结果表明，当微生物存在于生菜叶片上时，肠炎沙门氏菌SE86产生的生物表面活性剂有助于黏附并增加对消毒剂的抗性。
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