Saber-Samandari Samaneh, Saber-Samandari Saeed, Kiyazar Shiva, Aghazadeh Jamshid, Sadeghi Ali
Department of Chemistry, Eastern Mediterranean University, Gazimagusa, TRNC via Mersin 10, Turkey.
New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran.
Int J Biol Macromol. 2016 May;86:434-42. doi: 10.1016/j.ijbiomac.2016.01.102. Epub 2016 Feb 4.
Research on synthetic bioactive bone graft materials has significantly expanded in the past decade. In this study, the nanocomposite scaffold of semi-interpenetrating networks (semi-IPN) cellulose-graft-polyacrylamide/nano-hydroxyapatite was synthesized through free radical polymerization. The scaffolds were fabricated by the freeze-drying technique. The prepared semi-IPN nanocomposite scaffolds were characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD) analysis. In addition, the mechanical properties (i.e., elastic modulus and compressive strength) of the scaffolds were investigated. The SEM images showed that the pores of the scaffolds were interconnected, and their sizes ranged from 120 μm to 190 μm. Under optimum conditions, the prepared scaffolds had a compressive strength of 4.80 MPa, an elastic modulus of 0.29 GPa and a value of 47.37% porosity. Furthermore, the apatite-forming ability of the scaffolds was determined using simulated body fluid (SBF) for 28 days. The results revealed that the new apatite particles could grow on the surface of the scaffolds after a 14-day immersion in SBF. Finally, this study suggests that the prepared semi-IPN nanocomposites that closely mimic the properties of bone tissue could be a promising scaffold for bone tissue engineering.
在过去十年中，合成生物活性骨移植材料的研究有了显著扩展。在本研究中，通过自由基聚合合成了半互穿网络（semi-IPN）纤维素接枝聚丙烯酰胺/纳米羟基磷灰石的纳米复合支架。采用冷冻干燥技术制备支架。通过傅里叶变换红外（FTIR）光谱、扫描电子显微镜（SEM）和X射线衍射（XRD）分析对制备的半IPN纳米复合支架进行表征。此外，还研究了支架的力学性能（即弹性模量和抗压强度）。SEM图像显示，支架的孔隙相互连通，其尺寸范围为120μm至190μm。在最佳条件下，制备的支架抗压强度为4.80MPa，弹性模量为0.29GPa，孔隙率为47.37%。此外，使用模拟体液（SBF）测定支架的磷灰石形成能力，为期28天。结果表明，在SBF中浸泡14天后，新的磷灰石颗粒可以在支架表面生长。最后，本研究表明，所制备的紧密模拟骨组织特性的半IPN纳米复合材料可能是一种有前途的骨组织工程支架。
