Naulé Lydie, Marie-Luce Clarisse, Parmentier Caroline, Martini Mariangela, Albac Christelle, Trouillet Anne-Charlotte, Keller Matthieu, Hardin-Pouzet Hélène, Mhaouty-Kodja Sakina
Neuroscience Paris Seine, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche (UMR) S1130, Université P. et M. Curie, Paris, France; Centre National de la Recherche Scientifique, UMR 8246, Université P. et M. Curie, Paris, France; Sorbonne Universités, Université P. et M. Curie UM CR18, Université Paris 06, France.
UMR 85, Institut National de la Recherche Agronomique, Nouzilly, France; UMR7247, Centre National de la Recherche Scientifique, Nouzilly, France; Université François Rabelais, Tours, France; Institut Français du Cheval et de l'Equitation, Nouzilly, France.
Horm Behav. 2016 Apr;80:1-9. doi: 10.1016/j.yhbeh.2016.01.014. Epub 2016 Feb 1.
Estradiol derived from neural aromatization of gonadal testosterone plays a key role in the perinatal organization of the neural circuitry underlying male sexual behavior. The aim of this study was to investigate the contribution of neural estrogen receptor (ER) β in estradiol-induced effects without interfering with its peripheral functions. For this purpose, male mice lacking ERβ in the nervous system were generated. Analyses of males in two consecutive tests with a time interval of two weeks showed an effect of experience, but not of genotype, on the latencies to the first mount, intromission, pelvic thrusting and ejaculation. Similarly, there was an effect of experience, but not of genotype, on the number of thrusts and mating length. Neural ERβ deletion had no effect on the ability of males to adopt a lordosis posture in response to male mounts, after castration and priming with estradiol and progesterone. Indeed, only low percentages of both genotypes exhibited a low lordosis quotient. It also did not affect their olfactory preference. Quantification of tyrosine hydroxylase- and kisspeptin-immunoreactive neurons in the preoptic area showed unaffected sexual dimorphism of both populations in mutants. By contrast, the number of androgen receptor- and ERα-immunoreactive cells was significantly increased in the bed nucleus of stria terminalis of mutant males. These data show that neural ERβ does not play a crucial role in the organization and activation of the neural circuitry underlying male sexual behavior. These discrepancies with the phenotype of global ERβ knockout models are discussed.
由性腺睾酮经神经芳香化作用衍生而来的雌二醇，在雄性性行为潜在神经回路的围产期组织过程中发挥着关键作用。本研究的目的是探究神经雌激素受体（ER）β在雌二醇诱导效应中的作用，同时不干扰其外周功能。为此，构建了神经系统中缺乏ERβ的雄性小鼠。对雄性小鼠进行连续两次间隔两周的测试分析表明，经验而非基因型对首次爬跨、插入、骨盆推撞和射精的潜伏期有影响。同样，经验而非基因型对推撞次数和交配时长有影响。在阉割并用雌二醇和孕酮进行预处理后，神经ERβ缺失对雄性小鼠响应雄性爬跨而采取脊柱前凸姿势的能力没有影响。实际上，两种基因型中只有低比例个体表现出低脊柱前凸商数。它也不影响它们的嗅觉偏好。对视前区酪氨酸羟化酶和亲吻素免疫反应性神经元的定量分析表明，突变体中这两种神经元群体的性二态性未受影响。相比之下，突变体雄性小鼠终纹床核中雄激素受体和ERα免疫反应性细胞的数量显著增加。这些数据表明，神经ERβ在雄性性行为潜在神经回路的组织和激活过程中不发挥关键作用。本文讨论了这些与全局ERβ基因敲除模型表型的差异。
