Bariker Rajesh Hemant, Mandroli Praveenkumar S
Department of Pedodontics and Preventive Dentistry, Maratha Mandal's Nathajirao G. Halgekar Institution of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
J Indian Soc Pedod Prev Dent. 2016 Jan-Mar;34(1):23-9. doi: 10.4103/0970-4388.175506.
To evaluate the antibacterial action of Amalgomer CR and Fuji VII against bacteria causing S-early childhood caries.
Antibacterial activity of Amalgomer CR and Fuji VII was assessed using the agar diffusion test in triplicate. The powder and liquid of each test material was mixed and inserted in the punched wells (6 mm × 2 mm). A composition of 0.2% chlorhexidine digluconate acted as control. The agar plates were incubated at 37°C for 24 h for Streptococcus mutans, S. salivarius, S. parasanguinis and Actinomyces viscosus, whereas Lactobacillus casei was incubated for 48 h. Sizes of the inhibition zones were calculated by subtracting the diameter of the specimen (6 mm) from the average of the three measurements of the halo. For each test material against each bacteria, 9 measurements were made (3 measurements × 3 times). Kruskal-Wallis test was done to compare the zones of inhibition of test materials against individual bacteria. Pair-wise comparison was done by Mann-Whitney U-test.
Amalgomer CR had the most antibacterial against S. mutans (31.0 mm), followed by A. viscosus (21.87 mm), S. salivarius (13.87 mm), S. parasanguinis (10.80 mm), and L. casei (9.69 mm). Fuji VII had the most antibacterial action against S. salivarius (10.65 mm), followed by A. viscosus (9.10 mm). However, it did not inhibit the growth of S. mutans (0 mm), S. parasanguinis (0 mm), and L. casei (0 mm).
Amalgomer CR and Fuji VII showed wide variation in antibacterial action against all test organisms.
评估聚羧酸锌粘固粉CR和富士VII对引起儿童早期龋的细菌的抗菌作用。
采用琼脂扩散试验对聚羧酸锌粘固粉CR和富士VII的抗菌活性进行评估，重复三次。将每种测试材料的粉和液混合后，放入冲孔（6毫米×2毫米）中。以0.2%葡萄糖酸氯己定作为对照。琼脂平板在37°C下分别培养24小时，用于变形链球菌、唾液链球菌、副血链球菌和粘性放线菌，而干酪乳杆菌则培养48小时。通过从晕圈的三次测量平均值中减去标本直径（6毫米）来计算抑菌圈大小。对于每种测试材料针对每种细菌，进行9次测量（3次测量×3次）。采用Kruskal-Wallis检验比较测试材料对各细菌的抑菌圈。采用Mann-Whitney U检验进行两两比较。
聚羧酸锌粘固粉CR对变形链球菌的抗菌作用最强（31.0毫米），其次是粘性放线菌（21.87毫米）、唾液链球菌（13.87毫米）、副血链球菌（10.80毫米）和干酪乳杆菌（9.69毫米）。富士VII对唾液链球菌的抗菌作用最强（10.65毫米），其次是粘性放线菌（9.10毫米）。然而，它对变形链球菌（0毫米）、副血链球菌（0毫米）和干酪乳杆菌（0毫米）的生长没有抑制作用。
聚羧酸锌粘固粉CR和富士VII对所有测试微生物的抗菌作用差异很大。
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