Mione J, Manrique C, Duhoo Y, Roman F S, Guiraudie-Capraz G
Aix Marseille Université, CNRS, NICN, UMR 7259, 13344 Marseille, France.
Aix Marseille Université, CNRS, FR 3512, 13331 Marseille, France.
Neurobiol Learn Mem. 2016 Apr;130:52-60. doi: 10.1016/j.nlm.2016.01.011. Epub 2016 Feb 2.
Neuronal plasticity and neurogenesis occur in the adult hippocampus and in other brain structures such as the olfactory bulb and often involve the neural cell adhesion molecule NCAM. During an olfactory associative discrimination learning task, NCAM polysialylation triggers neuronal plasticity in the adult hippocampus. The PST enzyme likely modulates this polysialylation, but not STX, a second sialyltransferase. How the two polysialyltransferases are involved in the adult olfactory bulb remains unknown. We addressed this question by investigating the effect of olfactory associative learning on plasticity and neurogenesis. After a hippocampo-dependent olfactory associative task learning, we measured the expression of both PST and STX polysialyltransferases in the olfactory bulbs of adult rats using quantitative PCR. In parallel, immunohistochemistry was used to evaluate both NCAM polysialylation level and newly-born cells, with or without learning. After learning, no changes were observed neither in the expression level of PST and NCAM polysialylation, nor in STX gene expression level and newly-born cells number in the olfactory bulb.
神经元可塑性和神经发生在成年海马体以及其他脑结构（如嗅球）中发生，并且通常涉及神经细胞黏附分子NCAM。在嗅觉联想辨别学习任务期间，NCAM多唾液酸化触发成年海马体中的神经元可塑性。PST酶可能调节这种多唾液酸化，但不调节第二种唾液酸转移酶STX。这两种多唾液酸转移酶如何参与成年嗅球仍不清楚。我们通过研究嗅觉联想学习对可塑性和神经发生的影响来解决这个问题。在进行依赖海马体的嗅觉联想任务学习后，我们使用定量PCR测量成年大鼠嗅球中PST和STX多唾液酸转移酶的表达。同时，使用免疫组织化学评估有无学习情况下的NCAM多唾液酸化水平和新生细胞。学习后，嗅球中PST和NCAM多唾液酸化的表达水平、STX基因表达水平以及新生细胞数量均未观察到变化。
