[氨基糖苷类和氟喹诺酮类抗生素在细胞培养中的常见细胞毒性研究]

[Study of common cytotoxicity of aminoglycoside and fluoroquinolone antibiotics in cell cultures].

作者信息

Alexandrova O I, Khorolskaya Y I, Maychuk D Y, Blinova M I, Alexandrova O I, Khorolskaya Y I, Maychuk D Y, Blinova M I

机构信息

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064.

MNTK 'Eye Microsurgery' named after Acad. SN Fedorov, Beskudnikovsky Boulevard, 5a, Moscow, Russian Federation 127486.

出版信息

Vestn Oftalmol. 2015 Sep-Oct;131(5):43-53. doi: 10.17116/oftalma2015131543-53.

DOI:10.17116/oftalma2015131543-53

PMID:26845872

Abstract

UNLABELLED

The aim of the study was to identify the overall cytotoxicity of aminoglycosides netilmicin and tobramycin, and fluoroquinolone ciprofloxacin.

MATERIAL AND METHODS

We used three types of the cells: constantly transformed cell line CHO-K1, normal human skin fibroblasts and cells of normal human conjunctiva Clone 1-5C-4. Antibiotics activity was detected by their influence on cell viability. Quantitative and qualitative methods of evaluation have used to determine viability of the cells (quantitative assessment: a method of cloning cells and colorimetric method for assessing cell proliferation; qualitative assessment: lifetime visual observation under an inverted microscope for morphological status of cells in culture with photofixing).

RESULTS

The most toxic effect for all types of the cells was shown in tobramycin. The least degree of toxicity for all types of the cells was determined in netilmicin.

CONCLUSION

Studied antibiotics exert a cytotoxic effect in vitro and differ in their cytotoxic potential.

摘要

未标记

本研究的目的是确定氨基糖苷类药物奈替米星和妥布霉素以及氟喹诺酮类环丙沙星的总体细胞毒性。

材料与方法

我们使用了三种类型的细胞：持续转化的细胞系CHO-K1、正常人皮肤成纤维细胞和正常人结膜克隆1-5C-4细胞。通过抗生素对细胞活力的影响来检测其活性。采用定量和定性评估方法来确定细胞活力（定量评估：细胞克隆法和比色法评估细胞增殖；定性评估：在倒置显微镜下进行实时观察，对培养中的细胞形态状态进行光固定拍照）。