Papa Vincenzo, Leonardi Andrea, Getuli Claudia, Pacelli Vanessa, Russo Pietro, Milazzo Giovanni
Medical Department, SIFI S.p.A., Catania, Italy.
J Ocul Pharmacol Ther. 2003 Dec;19(6):535-45. doi: 10.1089/108076803322660459.
The purpose of this study was to compare the cytotoxic effects of the fluoroquinolone ofloxacin with that of the aminoglycoside netilmicin. Human corneal epithelial cells (HCE-T) and human conjunctival epithelial cells (Wong-Kilbourne derivative of Chang conjunctiva) were exposed to antibiotics (0.08-5.0 mg/mL) for 4 or 24 hours. Cell proliferation and viability were assessed with the MTT assay, neutral red uptake, and bromo deoxy uridine incorporation. In both cell lines, ofloxacin inhibited cell proliferation and viability. These effects were time and dose dependent. Concentrations of ofloxacin ranging from 0.4 to 2.4 mg/mL (0.04% to 0.24%) produced a 50% inhibition of proliferation and viability. In contrast, netilmicin induced no toxic effect. The differences between ofloxacin and netilmicin were highly statistically significant (p < 0.001). This finding is particularly relevant in deciding the optimal antibiotic to be applied in clinical situations in which the epithelium is compromised.
本研究的目的是比较氟喹诺酮类药物氧氟沙星与氨基糖苷类药物奈替米星的细胞毒性作用。将人角膜上皮细胞（HCE-T）和人结膜上皮细胞（张氏结膜的Wong-Kilbourne衍生物）暴露于抗生素（0.08 - 5.0 mg/mL）中4或24小时。通过MTT法、中性红摄取法和溴脱氧尿苷掺入法评估细胞增殖和活力。在两种细胞系中，氧氟沙星均抑制细胞增殖和活力。这些作用具有时间和剂量依赖性。氧氟沙星浓度在0.4至2.4 mg/mL（0.04%至0.24%）范围内可产生50%的增殖和活力抑制。相比之下，奈替米星未诱导毒性作用。氧氟沙星和奈替米星之间的差异具有高度统计学意义（p < 0.001）。这一发现对于决定在角膜上皮受损的临床情况下应用的最佳抗生素尤为重要。
