Zhang Y-G, Yang H-L, Long Y, Li W-L
Department of Clinical Laboratory, Guang Zhou Women and Children's Medical Centre of Guang Zhou Medical University, Guangzhou, Guangdong, China.
BJOG. 2016 Dec;123(13):2113-2118. doi: 10.1111/1471-0528.13897. Epub 2016 Feb 5.
To determine the expression of circular RNA (circRNA) in blood corpuscles of pregnant women before 20 weeks of pregnancy and to create a new model to identify the performance of circRNA combined with protein factors for the early diagnosis of pre-eclampsia (PE).
Nested case-control study.
University medical centre, Guangzhou, China.
A total of 1400 pregnant women recruited between 8 and 20 weeks of gestation. In all, 41 women with PE were included in the study, and were matched with 41 normally pregnant women based on maternal age and gestational age at same-size ratio.
The samples were analysed using a human circRNA microarray in the discovery phase, then the circRNA and the plasma protein factor endoglin (ENG) were validated. Finally we combined ENG with circRNA to create a new early prediction model for PE.
Early changes of circRNA and ENG in PE.
The circ_101222 levels in blood corpuscles of patients with PE were significantly higher than those in corresponding healthy women (P < 0.001). Using ENG in combination with circ_101222 resulted in a sensitivity of 0.7073, a specificity of 0.8049, and overall area under the curve of 0.876 (95% confidence interval 0.816-0.922) for the prediction of PE.
CircRNA and plasma proteins may have some predictive value for PE (such as circ_101222 and ENG). The performance of each of these factors may be strengthened when plasma proteins are used in combination with circRNA. The results are preliminary and need to be validated in larger studies and other populations.
Plasma protein endoglin in combination with circ_101222 strengthened the predictive power for pre-eclampsia.
确定妊娠20周前孕妇血细胞中环状RNA（circRNA）的表达情况，并建立一种新模型，以评估circRNA联合蛋白质因子用于子痫前期（PE）早期诊断的性能。
巢式病例对照研究。
中国广州的大学医学中心。
共招募1400例妊娠8至20周的孕妇。本研究共纳入41例PE患者，并根据产妇年龄和孕周按相同比例与41例正常孕妇进行匹配。
在探索阶段使用人circRNA微阵列对样本进行分析，然后对circRNA和血浆蛋白因子内皮糖蛋白（ENG）进行验证。最后，将ENG与circRNA联合，建立一种新的PE早期预测模型。
PE中circRNA和ENG的早期变化。
PE患者血细胞中circ_101222水平显著高于相应健康女性（P<0.001）。联合使用ENG和circ_101222预测PE时，灵敏度为0.7073，特异度为0.8049，曲线下总面积为0.876（95%置信区间0.816-0.922）。
CircRNA和血浆蛋白可能对PE具有一定预测价值（如circ_101222和ENG）。当血浆蛋白与circRNA联合使用时，这些因素各自的性能可能会增强。本结果为初步研究，需要在更大规模研究和其他人群中进行验证。
血浆蛋白内皮糖蛋白联合circ_101222增强了子痫前期的预测能力。
