Pansegrau Werner, Bagnoli Fabio
GSK Vaccines, Siena, Italy.
Curr Top Microbiol Immunol. 2017;404:203-233. doi: 10.1007/82_2015_5016.
Pili of Gram-positive bacteria are unique structures on the bacterial surface, assembled from covalently linked polypeptide subunits. Pilus assembly proceeds by transpeptidation reactions catalyzed by sortases, followed by covalent anchoring of the filament in the peptidoglycan layer. Another distinctive property is the presence of intramolecular isopeptide bonds, conferring extraordinary chemical and mechanical stability to these elongated structures. Besides their function in cell adhesion and biofilm formation, this section discusses possible application of pilus constituents as vaccine components against Gram-positive pathogens.
革兰氏阳性菌的菌毛是细菌表面独特的结构，由共价连接的多肽亚基组装而成。菌毛组装通过分选酶催化的转肽反应进行，随后细丝共价锚定在肽聚糖层中。另一个显著特性是分子内异肽键的存在，赋予这些细长结构非凡的化学和机械稳定性。除了在细胞黏附和生物膜形成中的作用外，本节还讨论了菌毛成分作为抗革兰氏阳性病原体疫苗成分的可能应用。
