酿酒酵母1号染色体DNA的克隆：FUN2转录区域的突变分析。

Cloning of chromosome I DNA from Saccharomyces cerevisiae: mutational analysis of the FUN2 transcribed region.

作者信息

Crowley J C, Kaback D B

机构信息

Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, Newark 07103.

出版信息

Gene. 1989 Nov 30;83(2):381-5. doi: 10.1016/0378-1119(89)90126-1.

DOI:10.1016/0378-1119(89)90126-1

PMID:2684793

Abstract

To increase the number of mutationally defined genes on chromosome I from Saccharomyces cerevisiae, most of the FUN2 (Function Unknown Now) transcribed region was deleted by gene replacement. Strains containing the deletion were viable, but grew with a 20% longer generation time. The mutation was recessive. Mutant haploids were able to mate, and homozygous mutant diploids were able to sporulate, giving asci containing four viable ascospores. These results indicate FUN2 is dispensable for the life cycle of S. cerevisiae, but required for an optimal growth rate.

摘要

为了增加酿酒酵母第一条染色体上经突变定义的基因数量，通过基因置换删除了大部分FUN2（目前功能未知）转录区域。含有该缺失的菌株能够存活，但生长时世代时间延长20%。该突变是隐性的。突变单倍体能够交配，纯合突变二倍体能够形成孢子，产生含有四个可存活子囊孢子的子囊。这些结果表明，FUN2对于酿酒酵母的生命周期不是必需的，但对于最佳生长速率是必需的。