Wang Zhifa, Li Zhijin, Dai Taiqiang, Zong Chunlin, Liu Yanpu, Liu Bin
State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
State Key Laboratory of Military Stomatology, Department of Oral Biology, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Int J Mol Sci. 2016 Feb 2;17(2):70. doi: 10.3390/ijms17020070.
To determine the effect of adipose-derived stem cells (ADSCs) added to bone marrow-derived mesenchymal stem cell (MSC) sheets on bone formation at an ectopic site. We isolated MSCs and ADSCs from the same rabbits. We then prepared MSC sheets for implantation with or without ADSCs subcutaneously in the backs of severe combined immunodeficiency (SCID) mice. We assessed bone formation at eight weeks after implantation by micro-computed tomography and histological analysis. In osteogenic medium, MSCs grew to form multilayer sheets containing many calcium nodules. MSC sheets without ADSCs formed bone-like tissue; although neo-bone and cartilage-like tissues were sparse and unevenly distributed by eight weeks after implantation. In comparison, MSC sheets with ADSCs promoted better bone regeneration as evidenced by the greater density of bone, increased mineral deposition, obvious formation of blood vessels, large number of interconnected ossified trabeculae and woven bone structures, and greater bone volume/total volume within the composite constructs. Our results indicate that although sheets of only MSCs have the potential to form tissue engineered bone at an ectopic site, the addition of ADSCs can significantly increase the osteogenic potential of MSC sheets. Thus, the combination of MSC sheets with ADSCs may be regarded as a promising therapeutic strategy to stimulate bone regeneration.
为了确定添加到骨髓间充质干细胞（MSC）片层中的脂肪来源干细胞（ADSC）对异位部位骨形成的影响。我们从同一只兔子身上分离出MSC和ADSC。然后，我们制备了用于植入的MSC片层，将其皮下植入严重联合免疫缺陷（SCID）小鼠的背部，植入时添加或不添加ADSC。我们通过微计算机断层扫描和组织学分析评估植入后八周时的骨形成情况。在成骨培养基中，MSC生长形成含有许多钙结节的多层片层。不添加ADSC的MSC片层形成了类骨组织；尽管在植入后八周时新生骨和软骨样组织稀疏且分布不均。相比之下，添加ADSC的MSC片层促进了更好的骨再生，这表现为骨密度更高、矿物质沉积增加、血管明显形成、大量相互连接的骨小梁和编织骨结构，以及复合构建物中更大的骨体积/总体积。我们的结果表明，尽管仅MSC片层有在异位部位形成组织工程骨的潜力，但添加ADSC可显著增加MSC片层的成骨潜力。因此，MSC片层与ADSC的组合可被视为一种有前景的促进骨再生的治疗策略。
