Ozgun E, Ozgun G S, Gokmen S S, Eskıocak S, Sut N, Akıncı M, Goncu E, Cakır E
Medical Biochemistry, Trakya University, Edirne, Turkey.
Biostatistics and Medical Informatics, Trakya University, Edirne, Turkey.
Exp Clin Endocrinol Diabetes. 2019 Jun;127(6):377-384. doi: 10.1055/s-0042-101164. Epub 2016 Feb 5.
The aim of the present study was to investigate the effect of streptozotocin-induced diabetes mellitus and lipoic acid treatment on serum paraoxonase-1 and paraoxonase-3 protein levels and paraoxonase, arylesterase and lactonase activities.36 rats were equally and randomly divided into 4 groups as control, lipoic acid, diabetes and diabetes+lipoic acid. To induce diabetes, a single dose of streptozotocin (40 mg/kg) was injected intraperitoneally to diabetes and diabetes+lipoic acid groups. Lipoic acid (10 mg/kg/day) was injected intraperitoneally for 14 days to lipoic acid and diabetes+lipoic acid groups. Serum PON1 and PON3 protein levels were measured by western blotting. Serum paraoxonase, arylesterase and lactonase activities were determined by the measuring initial rate of substrate (paraoxon, phenylacetate and dihydrocoumarin) hydrolysis.Streptozotocin-induced diabetes mellitus caused a significant decrease whereas lipoic acid treatment caused a significant increase in serum PON1 and PON3 protein levels and paraoxonase, arylesterase and lactonase activities. The increase percent of serum PON3 protein was higher than that of serum PON1 protein and the increase percent of serum lactonase activity was higher than that of serum paraoxonase and arylesterase activities in diabetes+lipoic acid group.We can report that, like PON1 protein, PON3 protein and actually its lactonase activity may also have a role as an antioxidant in diabetes mellitus and lipoic acid treatment may be useful for the prevention of the atherosclerotic complications of diabetes by increasing serum PON1 and PON3 protein levels and serum enzyme activities.
本研究的目的是调查链脲佐菌素诱导的糖尿病以及硫辛酸治疗对血清对氧磷酶-1和对氧磷酶-3蛋白水平以及对氧磷酶、芳基酯酶和内酯酶活性的影响。36只大鼠被平均随机分为4组，即对照组、硫辛酸组、糖尿病组和糖尿病+硫辛酸组。为诱导糖尿病，向糖尿病组和糖尿病+硫辛酸组腹腔注射单剂量链脲佐菌素（40mg/kg）。向硫辛酸组和糖尿病+硫辛酸组腹腔注射硫辛酸（10mg/kg/天），持续14天。通过蛋白质印迹法测量血清对氧磷酶1（PON1）和对氧磷酶3（PON3）蛋白水平。通过测量底物（对氧磷、苯乙酸和二氢香豆素）水解的初始速率来测定血清对氧磷酶、芳基酯酶和内酯酶活性。链脲佐菌素诱导的糖尿病导致血清PON1和PON3蛋白水平以及对氧磷酶、芳基酯酶和内酯酶活性显著降低，而硫辛酸治疗则导致显著升高。在糖尿病+硫辛酸组中，血清PON3蛋白的升高百分比高于血清PON1蛋白，血清内酯酶活性的升高百分比高于血清对氧磷酶和芳基酯酶活性。我们可以报告，与PON1蛋白一样，PON3蛋白及其内酯酶活性实际上在糖尿病中也可能作为抗氧化剂发挥作用，硫辛酸治疗可能通过提高血清PON1和PON3蛋白水平以及血清酶活性，对预防糖尿病的动脉粥样硬化并发症有用。
