Ferretti V A, Segal-Eiras A, Barbeito C G, Croce M V
Centre of Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, La Plata, Argentina.
Laboratory of Histology and Embryology, Faculty of Veterinary Sciences, National University of La Plata, Argentina.
Res Vet Sci. 2016 Feb;104:136-45. doi: 10.1016/j.rvsc.2015.12.012. Epub 2015 Dec 23.
Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells of small and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults.
分泌型粘蛋白是保护呼吸道和消化道上皮的凝胶的关键组成部分，MUC2/Muc2是肠道中主要的凝胶形成粘蛋白，而MUC5AC/Muc5ac是气道中表达最丰富的凝胶形成粘蛋白之一。在本研究中，我们通过免疫组织化学、蛋白质印迹和逆转录聚合酶链反应分析了大鼠发育过程中的Muc2和Muc5ac。我们证明，大鼠Muc2在胎儿小肠绒毛表面上皮和发育中的利伯库恩隐窝的杯状细胞中表达。在新生儿和成年人中，Muc2在小肠和大肠的管腔杯状细胞以及胃粘液和腺细胞中表达。在胚胎期的胃和肺样本中观察到Muc5ac蛋白；其表达在发育过程以及出生后和成年期增加。出生后，在气管表面上皮和腺体中检测到低反应，在成年人中增加。
