Ellepola Arjuna Nishantha Bandara, Chandy Rachel, Khan Zia Uddin, Samaranayake Lakshman Perera
Department of Bioclinical Sciences, Faculty of Dentistry.
Department of Microbiology, Faculty of Medicine, Health Sciences Center, Kuwait University, Kuwait.
Microbiol Immunol. 2016 Mar;60(3):160-7. doi: 10.1111/1348-0421.12362.
Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulence traits involved in the pathogenicity of Candida. Post-antifungal effect (PAFE) also have a bearing on pathogenicity and virulence of Candida. Candida dubliniensis is associated with oral and systemic candidosis, which can be managed with caspofungin. There is no published information on caspofungin-induced PAFE and its impact on adhesion traits of C. dubliniensis isolates. Thus, the purpose of this investigation was to determine the in vitro duration of PAFE on 20 C. dubliniensis isolates following transient exposure to caspofungin. Furthermore the impacts of caspofungin-induced PAFE on adhesion to BEC and DAS, GT formation and CSH of these isolates were also determined. After establishing the minimum inhibitory concentration (MIC) of caspofungin, C. dubliniensis isolates were exposed to sub-lethal concentrations (×3 MIC) of caspofungin for 1 hr. Thereafter the duration of PAFE, adhesion to BEC and DAS, GT formation and CSH were determined by previously described in-vitro assays. MIC (μg/mL) of C. dubliniensis isolates to caspofungin ranged from 0.004 to 0.19. Caspofungin-induced mean PAFE on C. dubliniensis isolates was 2.17 hr. Exposure to caspofungin suppressed the ability of C. dubliniensis isolates to adhere to BEC and DAS, form GT and CSH by 69.97%, 71.95%, 90.06% and 32.29% (P < 0.001 for all), respectively. Thus, transient exposure of C. dubliniensis isolates to caspofungin produces an antifungal effect not only by suppressing its growth but also by altering its adhesion traits.
对颊上皮细胞（BEC）和义齿丙烯酸表面（DAS）的黏附、芽管（GT）形成以及细胞表面疏水性（CSH）都是白色念珠菌致病性所涉及的毒力特征。抗真菌后效应（PAFE）也与白色念珠菌的致病性和毒力有关。都柏林念珠菌与口腔和全身性念珠菌病相关，可使用卡泊芬净进行治疗。关于卡泊芬净诱导的PAFE及其对都柏林念珠菌分离株黏附特性的影响，目前尚无公开信息。因此，本研究的目的是确定短暂暴露于卡泊芬净后，PAFE对20株都柏林念珠菌分离株的体外持续时间。此外，还确定了卡泊芬净诱导的PAFE对这些分离株黏附于BEC和DAS、GT形成以及CSH的影响。在确定卡泊芬净的最低抑菌浓度（MIC）后，将都柏林念珠菌分离株暴露于亚致死浓度（×3 MIC）的卡泊芬净中1小时。此后，通过先前描述的体外试验确定PAFE的持续时间、对BEC和DAS的黏附、GT形成以及CSH。都柏林念珠菌分离株对卡泊芬净的MIC（μg/mL）范围为0.004至0.19。卡泊芬净对都柏林念珠菌分离株诱导的平均PAFE为2.17小时。暴露于卡泊芬净后，都柏林念珠菌分离株黏附于BEC和DAS、形成GT和CSH的能力分别被抑制了69.97%、71.95%、90.06%和32.29%（所有P均<0.001）。因此，都柏林念珠菌分离株短暂暴露于卡泊芬净不仅通过抑制其生长产生抗真菌作用，还通过改变其黏附特性发挥作用。
