Yazici-Tutunis Secil, Gurel-Gurevin Ebru, Ustunova Savas, Demirci-Tansel Cihan, Mericli Filiz
a Department of Pharmacognosy, Faculty of Pharmacy , Istanbul University , Istanbul , Turkey ;
b Department of Biology, Faculty of Science , Istanbul University , Istanbul , Turkey ;
Pharm Biol. 2016 Oct;54(10):1991-7. doi: 10.3109/13880209.2015.1137952. Epub 2016 Feb 5.
Context Phillyrea latifolia L. (Oleaceae), commonly found in the Mediterranean region in Turkey, is used as medicinal teas for weight loss and hyperglycaemia in folk medicine. Objective The study investigated the possible effects of P. latifolia leaves aqueous extract's on weight loss and biochemical-histological changes in the rats fed a high-energy diet (HED), also isolated and determined the main phenolic compounds. Materials and methods Twenty-four male Wistar albino rats were divided into four equal groups such as the HED group fed a HED, the PLE group given only the extract of P. latifolia leaves (220 mg/kg), the HED + PLE group administrated with the extract of leaves (220 mg/kg) after being fed with HED and a control group fed with standard pellet diet. Results PLE administration caused a remarkable decrement of body weight in the HED + PLE group (p < 0.05). PLE showed an improved effect on structural integrity and decreased leukocyte infiltration in liver and small intestinal tissues. The blood glucose (117.3 mmol/L), leptin (5.6 ng/mL), total cholesterol (61.8 mg/dL) and LDL (9.3 mmol/L) levels were significantly increased in the HED group. PLE administration in the HED group decreased these levels. The levels of HDL (26.8 mmol/L) in the HED + PLE group were higher than both control and HED groups. Chemical composition was investigated and luteolin 7-O-glucoside and chlorogenic acid were determined for the first time in Turkish sample from the EtOAc extract of leaves. Discussion and conclusion Phillyrea latifolia leaves may have beneficial effects on obesity related cellular problems and may become a good source of antidiabetic medication.
背景 地中海荚蒾（木犀科）常见于土耳其的地中海地区，在民间医学中被用作减肥和降血糖的药茶。目的 本研究调查了地中海荚蒾叶水提取物对高能饮食（HED）喂养大鼠体重减轻及生化组织学变化的可能影响，并分离鉴定了主要酚类化合物。材料与方法 将24只雄性Wistar白化大鼠分为四组，即喂食HED的HED组、仅给予地中海荚蒾叶提取物（220mg/kg）的PLE组、喂食HED后给予叶提取物（220mg/kg）的HED+PLE组以及喂食标准颗粒饲料的对照组。结果 在HED+PLE组中，给予PLE导致体重显著下降（p<0.05）。PLE对肝脏和小肠组织的结构完整性有改善作用，并减少了白细胞浸润。HED组的血糖（117.3mmol/L）、瘦素（5.6ng/mL）、总胆固醇（61.8mg/dL）和低密度脂蛋白（9.3mmol/L）水平显著升高。在HED组中给予PLE可降低这些水平。HED+PLE组的高密度脂蛋白（26.8mmol/L）水平高于对照组和HED组。对化学成分进行了研究，首次从叶的乙酸乙酯提取物的土耳其样品中测定了木犀草素7-O-葡萄糖苷和绿原酸。讨论与结论 地中海荚蒾叶可能对肥胖相关的细胞问题具有有益作用，可能成为抗糖尿病药物的良好来源。
