Parim BrahmaNaidu, Harishankar Nemani, Balaji Meriga, Pothana Sailaja, Sajjalaguddam Ramgopal Rao
Department of Biochemistry, Sri Venkateswara University , Tirupati, Andhra Pradesh , India .
Pharm Biol. 2015;53(9):1318-28. doi: 10.3109/13880209.2014.980585. Epub 2015 Apr 9.
Piper nigrum Linn (Piperaceae) (PnL) is used in traditional medicine to treat gastric ailments, dyslipidemia, diabetes, and hypertension.
The present study explores the possible protective effects of P. nigrum extracts on high-fat diet-induced obesity in rats.
High-fat diet-induced obese rats were treated orally with 200 mg/kg bw of different extracts (hexane, ethylacetate, ethanol, and aqueous extracts) of PnL for 42 d. The effects of PnL extracts on body composition, insulin resistance, biochemical parameters, leptin, adiponectin, lipid profile, liver marker enzymes, and antioxidants were studied.
The HFD control group rats showed a substantial raise in body weight (472.8 ± 9.3 g), fat% (20.8 ± 0.6%), and fat-free mass (165.9 ± 2.4 g) when compared with normal control rats whose body weight, fat%, and fat-free mass were 314.3 ± 4.4 g, 6.4 ± 1.4%, and 133.8 ± 2.2 g, respectively. Oral administration of ethyl acetate or aqueous extracts of PnL markedly reduced the body weight, fat%, and fat-free mass of HFD-fed rats. In contrast to the normal control group, a profound increase in plasma glucose, insulin resistance, lipid profile, leptin, thiobarbituric acid reactive substance (TBARS), and the activities of lipase and liver marker enzymes, and a decrease in adiponectin and antioxidant enzymes were noted in HFD control rats. Administration of PnL extracts to HFD-induced obese rats significantly (p < 0.05) restored the above profiles.
PnL extracts significantly reduced the body weight, fat%, and ameliorated HFD-induced hyperlipidemia and its constituents.
胡椒（胡椒科）在传统医学中用于治疗胃部疾病、血脂异常、糖尿病和高血压。
本研究探讨胡椒提取物对高脂饮食诱导的大鼠肥胖的可能保护作用。
用200mg/kg体重的胡椒不同提取物（己烷、乙酸乙酯、乙醇和水提取物）对高脂饮食诱导的肥胖大鼠进行口服给药，持续42天。研究了胡椒提取物对身体组成、胰岛素抵抗、生化参数、瘦素、脂联素、血脂谱、肝脏标志物酶和抗氧化剂的影响。
与正常对照组大鼠相比，高脂饮食对照组大鼠的体重（472.8±9.3g）、脂肪百分比（20.8±0.6%）和去脂体重（165.9±2.4g）显著增加，正常对照组大鼠的体重、脂肪百分比和去脂体重分别为314.3±4.4g、6.4±1.4%和133.8±2.2g。口服胡椒乙酸乙酯提取物或水提取物可显著降低高脂饮食喂养大鼠的体重、脂肪百分比和去脂体重。与正常对照组相比，高脂饮食对照组大鼠的血糖、胰岛素抵抗、血脂谱、瘦素、硫代巴比妥酸反应物质（TBARS）以及脂肪酶和肝脏标志物酶的活性显著升高，脂联素和抗氧化酶降低。给高脂饮食诱导的肥胖大鼠服用胡椒提取物可显著（p<0.05）恢复上述指标。
胡椒提取物显著降低了体重、脂肪百分比，并改善了高脂饮食诱导的高脂血症及其成分。
