维生素 D3 对结核分枝杆菌处理的树突状细胞成熟和抗原呈递功能的影响。

Effect of vitamin D3 on maturation and antigen-presenting function of dendritic cells treated with Mycobacterium tuberculosis.

机构信息

No 202 Hospital of People's Liberation Army, Liaoning 110003, China.

Department of Orthopedics, Southwest Hospital, Third Military Medical, Chongqing 400038, China.

出版信息

Asian Pac J Trop Med. 2016 Jan;9(1):54-7. doi: 10.1016/j.apjtm.2015.12.011. Epub 2015 Dec 19.

DOI:10.1016/j.apjtm.2015.12.011

PMID:26851787

Abstract

OBJECTIVE

To investigate the phenotypic characteristics and functional capability differences of mouse bone marrow-derived dendritic cells after stimulation with Mycobacterium tuberculosis in the presence or absence of vitamin D3.

METHODS

Mouse bone marrow-derived cells were cultured with GM-CSF (20 ng/mL). Then, one was added with 100 nmol/L of 25(OH)D3, while the other did not. On day 6, 5 μg/mL of BCG was added to stimulate the cells for 24 h. On day 7, suspension cells were harvested for phenotypic and functional analyses.

RESULTS

The percentages of CD86 dendritic cells (DCs) in the control group and 25(OH)D3 group were 66.97% ± 8.29% and 52.18% ± 8.52%, respectively; the mean fluorescence intensities of MHC-II in the control group and 25(OH)D3 group were 1 102.16 ± 371.02 and 681.62 ± 292.71. The expression levels of MHC- II and CD86 on the surface of the DCs in 25(OH)D3 group were significantly lower than those of the control group. The ability of the DCs to stimulate proliferation of T-lymphocytes was also significantly lower than that of the control group.

CONCLUSIONS

These findings suggest that 25(OH)D3 modulates the immune response by affecting the maturation and function of DCs in Mycobacterium tuberculosis period.

摘要

目的

研究在维生素 D3 存在或不存在的情况下，结核分枝杆菌刺激小鼠骨髓来源树突状细胞后的表型特征和功能能力差异。

方法

用 GM-CSF（20ng/mL）培养小鼠骨髓细胞。然后，一组加入 100nmol/L 的 25（OH）D3，另一组不加。第 6 天，加入 5μg/mL 的 BCG 刺激细胞 24 小时。第 7 天，收获悬浮细胞进行表型和功能分析。

结果

对照组和 25（OH）D3 组的 CD86 树突状细胞（DC）的百分比分别为 66.97%±8.29%和 52.18%±8.52%；对照组和 25（OH）D3 组 MHC-II 的平均荧光强度分别为 1102.16±371.02 和 681.62±292.71。25（OH）D3 组 DC 表面 MHC-II 和 CD86 的表达水平明显低于对照组。25（OH）D3 组 DC 刺激 T 淋巴细胞增殖的能力也明显低于对照组。