变形虫纲代表动物线粒体外膜中的蛋白质输入复合体。
Protein import complexes in the mitochondrial outer membrane of Amoebozoa representatives.
作者信息
Buczek Dorota, Wojtkowska Małgorzata, Suzuki Yutaka, Sonobe Seiji, Nishigami Yukinori, Antoniewicz Monika, Kmita Hanna, Makałowski Wojciech
机构信息
Institute of Bioinformatics, Faculty of Medicine, University of Muenster, Niels Stensen Strasse 14, 48149, Muenster, Germany.
Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland.
出版信息
BMC Genomics. 2016 Feb 6;17:99. doi: 10.1186/s12864-016-2402-2.
BACKGROUND
An ancestral trait of eukaryotic cells is the presence of mitochondria as an essential element for function and survival. Proper functioning of mitochondria depends on the import of nearly all proteins that is performed by complexes located in both mitochondrial membranes. The complexes have been proposed to contain subunits formed by proteins common to all eukaryotes and additional subunits regarded as lineage specific. Since Amoebozoa is poorly sampled for the complexes we investigated the outer membrane complexes, namely TOM, TOB/SAM and ERMES complexes, using available genome and transcriptome sequences, including transcriptomes assembled by us.
RESULTS
The results indicate differences in the organization of the Amoebozoa TOM, TOB/SAM and ERMES complexes, with the TOM complex appearing to be the most diverse. This is reflected by differences in the number of involved subunits and in similarities to the cognate proteins of representatives from different supergroups of eukaryotes.
CONCLUSIONS
The obtained results clearly demonstrate structural variability/diversity of these complexes in the Amoebozoa lineage and the reduction of their complexity as compared with the same complexes of model organisms.
背景
真核细胞的一个祖先特征是存在线粒体，这是其功能和生存的关键要素。线粒体的正常功能依赖于几乎所有蛋白质的导入，这一过程由位于线粒体内外膜的复合物完成。有人提出，这些复合物包含由所有真核生物共有的蛋白质形成的亚基以及被视为特定谱系的其他亚基。由于变形虫门在这些复合物方面的样本不足，我们利用现有的基因组和转录组序列，包括我们组装的转录组，对线粒体外膜复合物，即转位酶外膜复合物（TOM）、β桶组装机器/分选和组装机器（TOB/SAM）以及内质网-线粒体接触位点与嵴组织系统（ERMES）复合物进行了研究。
结果
结果表明，变形虫门的TOM、TOB/SAM和ERMES复合物在组织上存在差异，其中TOM复合物似乎最为多样。这体现在所涉及亚基数量的差异以及与不同真核生物超群代表的同源蛋白的相似性上。
结论
所获得的结果清楚地表明，在变形虫门谱系中这些复合物存在结构变异性/多样性，并且与模式生物的相同复合物相比，其复杂性有所降低。
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