Jäger J, Köhler E, Tucker P, Sauder U, Housley-Markovic Z, Fotheringham I, Edwards M, Hunter M, Kirschner K, Jansonius J N
Department of Structural Biology, University of Basel, Switzerland.
J Mol Biol. 1989 Oct 5;209(3):499-501. doi: 10.1016/0022-2836(89)90014-4.
Mutant aspartate aminotransferase V39L (Val39 replaced by Leu) from Escherichia coli has been crystallized into a monoclinic cell from a polyethylene glycol solution (pH 7.5) by vapor diffusion. The space group and the unit cell dimensions have been determined using a precession camera, a CAD4 diffractometer and a Nicolet Xentronics area detector to be P2(1) with a = 86.8 A, b = 79.9 A, c = 89.4 A, beta = 118.74 degrees. The crystals diffract to better than 2.3 A and are suitable for X-ray structure analysis.
来自大肠杆菌的突变天冬氨酸转氨酶V39L（缬氨酸39被亮氨酸取代）已通过气相扩散法，从聚乙二醇溶液（pH 7.5）中结晶到单斜晶胞中。使用进动相机、CAD4衍射仪和Nicolet Xentronics面探测器确定其空间群和晶胞尺寸为P2(1)，a = 86.8 Å，b = 79.9 Å，c = 89.4 Å，β = 118.74°。这些晶体的衍射分辨率优于2.3 Å，适合进行X射线结构分析。
