Crystallization and preliminary X-ray studies of an aspartate aminotransferase mutant from Escherichia coli.

作者信息

Jäger J, Köhler E, Tucker P, Sauder U, Housley-Markovic Z, Fotheringham I, Edwards M, Hunter M, Kirschner K, Jansonius J N

机构信息

Department of Structural Biology, University of Basel, Switzerland.

出版信息

J Mol Biol. 1989 Oct 5;209(3):499-501. doi: 10.1016/0022-2836(89)90014-4.

DOI:10.1016/0022-2836(89)90014-4

PMID:2685322

Abstract

Mutant aspartate aminotransferase V39L (Val39 replaced by Leu) from Escherichia coli has been crystallized into a monoclinic cell from a polyethylene glycol solution (pH 7.5) by vapor diffusion. The space group and the unit cell dimensions have been determined using a precession camera, a CAD4 diffractometer and a Nicolet Xentronics area detector to be P2(1) with a = 86.8 A, b = 79.9 A, c = 89.4 A, beta = 118.74 degrees. The crystals diffract to better than 2.3 A and are suitable for X-ray structure analysis.

摘要