RUNX3甲基化与喉鳞状细胞癌临床结局及细胞迁移/侵袭的相关性
Association of RUNX3 Methylation with Clinical Outcome and Cell Migration/Invasion in Laryngeal Squamous Cell Carcinoma.
作者信息
Liu Zhao-Hui, Liu Jia-Jia, Li Shi-Sheng, Yang Xin-Ming
机构信息
a Department of Otolaryngology, Head, and Neck Surgery , the Second Xiangya Hospital of Central South University , Hunan , China.
b Department of Otolaryngology, Head, and Neck Surgery , the Affiliated Hospital of Zunyi Medical College , Guizhou , China.
出版信息
Cancer Invest. 2016;34(2):105-13. doi: 10.3109/07357907.2015.1104690. Epub 2016 Feb 6.
This study identifies promoter methylation status of RUNX3 in 77 LSCC patient tissues and their paired adjacent healthy tissues. Hypermethylation percentage RUNX3 occurred in LSCC samples was significantly higher than that in normal tissues, as well as associated with suppression of RUNX3 expression, TNM classification of malignant tumors stage, lymph node metastasis and poor overall survival rate. Reduced methylation and increased expression of RUNX3 genes in vitro was observed and decreased cell migration was further confirmed following 5-azacytidine treatment. RUNX 3 promoter hypermethylation can lead to down-regulation of RUNX3 in LSCC cancerous tissue.
本研究鉴定了77例喉鳞状细胞癌（LSCC）患者组织及其配对的相邻健康组织中RUNX3的启动子甲基化状态。LSCC样本中RUNX3的高甲基化百分比显著高于正常组织，并且与RUNX3表达的抑制、恶性肿瘤的TNM分期、淋巴结转移及总体生存率低相关。体外观察到RUNX3基因甲基化降低且表达增加，5-氮杂胞苷处理后进一步证实细胞迁移减少。RUNX3启动子高甲基化可导致LSCC癌组织中RUNX3下调。
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