[Runx3基因高甲基化与喉癌发生发展及转移的关系]

[Relationship between hypermethylation of Runx3 gene and the development and metastasis of laryngeal carcinoma].

作者信息

Tang Jing, Bai Weiliang, Ji Wenyue, Gao Hong, Liu Jiao

机构信息

Department of Otorhinolaryngology, the Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Sep;24(18):834-7.

PMID:21254652

Abstract

OBJECTIVE

To explore the relationships between hypermethylation of human runt-related transcription factor 3 (Runx3) gene promoter and laryngeal squamous cell cancer.

METHOD

Promoter hypermethylation and mRNA expression were detected by methylation-specific PCR and RT-PCR.

RESULT

The expression of Runx3 gene mRNA detected in laryngeal carcinoma (1.62 +/- 1.01) was lower than that in adjacent tissues samples (5.66 +/- 2.07) (t = 10.72, P < 0.01). No methylation of Runx3 promoter was found in adjacent tissues samples. But hypermethylation was found in 95.0% (38/40) of the laryngeal carcinoma specimens. The rate of methylation of Runx3 promoter in laryngeal carcinoma was higher than that in adjacent tissues (P < 0.01). The Runx3 mRNA were down-regulated in lymphnode metastasis or poorly differentiated groups, but the Runx3 promoter methylation were detected in those groups markedly.

CONCLUSION

Hypermethylation of Runx3 promoter is one of the inactivation re-seasons in laryngeal squamous cell carcinoma, and the decreasing of Runx3 mRNA expression may be related to lymph node metastasis and development of laryngeal squamous cell carcinoma.

摘要

目的

探讨人 runt 相关转录因子 3（Runx3）基因启动子高甲基化与喉鳞状细胞癌之间的关系。

方法

采用甲基化特异性 PCR 和 RT-PCR 检测启动子高甲基化和 mRNA 表达。

结果

喉癌组织中 Runx3 基因 mRNA 表达量（1.62±1.01）低于癌旁组织（5.66±2.07）（t = 10.72，P < 0.01）。癌旁组织样本中未发现 Runx3 启动子甲基化。但在 95.0%（38/40）的喉癌标本中发现高甲基化。喉癌中 Runx3 启动子甲基化率高于癌旁组织（P < 0.01）。Runx3 mRNA 在淋巴结转移或低分化组中表达下调，但在这些组中明显检测到 Runx3 启动子甲基化。