[Complementary addressed alkylation of 16s rRNA from Escherichia coli by 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivatives of oligodeoxyribonucleotides. III. Segments of 16s rRNA interacting with the benzylidene derivative of d(pACCTTGTT)rA].

16S rRNA chain was cleaved by RNase H within covalent adduct with a 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivative of d(pACCTTGTT)rA. It was shown that no less than 50% of cleavage occurs at the 1498-1506 site. The selectivity of alkylation and correspondingly the cleavage by RNase H, at this site practically does not increase when RNA was alkylated to a low extent, and also when a small excess of the reagent in respect to rRNA was used.