McNulty Samantha N, Strübe Christina, Rosa Bruce A, Martin John C, Tyagi Rahul, Choi Young-Jun, Wang Qi, Hallsworth Pepin Kymberlie, Zhang Xu, Ozersky Philip, Wilson Richard K, Sternberg Paul W, Gasser Robin B, Mitreva Makedonka
The McDonnell Genome Institute, Washington University in St Louis, MO 63108, USA.
Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover 30559, Germany.
Sci Rep. 2016 Feb 9;6:20316. doi: 10.1038/srep20316.
The bovine lungworm, Dictyocaulus viviparus (order Strongylida), is an important parasite of livestock that causes substantial economic and production losses worldwide. Here we report the draft genome, variome, and developmental transcriptome of D. viviparus. The genome (161 Mb) is smaller than those of related bursate nematodes and encodes fewer proteins (14,171 total). In the first genome-wide assessment of genomic variation in any parasitic nematode, we found a high degree of sequence variability in proteins predicted to be involved host-parasite interactions. Next, we used extensive RNA sequence data to track gene transcription across the life cycle of D. viviparus, and identified genes that might be important in nematode development and parasitism. Finally, we predicted genes that could be vital in host-parasite interactions, genes that could serve as drug targets, and putative RNAi effectors with a view to developing functional genomic tools. This extensive, well-curated dataset should provide a basis for developing new anthelmintics, vaccines, and improved diagnostic tests and serve as a platform for future investigations of drug resistance and epidemiology of the bovine lungworm and related nematodes.
牛肺线虫，胎生网尾线虫（圆线目），是一种重要的家畜寄生虫，在全球范围内造成了巨大的经济和生产损失。在此，我们报告了胎生网尾线虫的基因组草图、变异组和发育转录组。其基因组（161 Mb）比相关有囊线虫的基因组小，编码的蛋白质也更少（总共14,171个）。在对任何寄生线虫基因组变异进行的首次全基因组评估中，我们发现预测参与宿主 - 寄生虫相互作用的蛋白质存在高度的序列变异性。接下来，我们利用大量的RNA序列数据追踪胎生网尾线虫整个生命周期中的基因转录，并鉴定了可能在线虫发育和寄生过程中起重要作用的基因。最后，我们预测了在宿主 - 寄生虫相互作用中可能至关重要的基因、可作为药物靶点的基因以及假定的RNA干扰效应物，以期开发功能基因组工具。这个广泛且精心整理的数据集应为开发新的驱虫药、疫苗以及改进诊断测试提供基础，并作为未来研究牛肺线虫及相关线虫耐药性和流行病学的平台。
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