Tang Hongbo, Luo Xinggu, Li Juan, Zhou Yi, Li Yanmei, Song Lijuan, Zhang Xiaowen, Chen Tao
Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of University of South China, Hengyang, Hunan, 421001, People's Republic China.
State Key Laboratory of Respiratory Disease, Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of Guangzhou Medical University, 151# West Yanjiang Road, Guangzhou, Guangdong, 510120, People's Republic China.
Tumour Biol. 2016 Aug;37(8):10563-9. doi: 10.1007/s13277-016-4922-4. Epub 2016 Feb 8.
Metabolism alteration is one of the hallmarks of cancer cells. Although several studies have demonstrated that glycolysis played important roles in the progression of nasopharyngeal carcinoma (NPC), the functions of specific metabolism-associated genes remain largely unknown. In this study, it was found that Pyruvate dehydrogenase B (PDHB), which catalyzed the conversion of pyruvate to Acetyl-CoA, was downregulated in NPC cells. Forced expression of PDHB in NPC cells inhibited cell growth and migration, while knocking down the expression of PDHB promoted the growth, migration, and tumorigenesis of NPC cells. Mechanism study showed that PDHB inhibited ERK signaling and cell growth driven by RasV12. Collectively, our study demonstrated the suppressive roles of PDHB in the progression of NPC, and restoring the function of PDHB might be a promising strategy for NPC therapy.
代谢改变是癌细胞的特征之一。尽管多项研究表明糖酵解在鼻咽癌（NPC）进展中起重要作用，但特定代谢相关基因的功能仍 largely 未知。在本研究中，发现催化丙酮酸转化为乙酰辅酶 A 的丙酮酸脱氢酶 B（PDHB）在 NPC 细胞中下调。在 NPC 细胞中强制表达 PDHB 可抑制细胞生长和迁移，而敲低 PDHB 的表达则促进 NPC 细胞的生长、迁移和肿瘤发生。机制研究表明，PDHB 抑制 ERK 信号传导和由 RasV12 驱动的细胞生长。总体而言，我们的研究证明了 PDHB 在 NPC 进展中的抑制作用，恢复 PDHB 的功能可能是 NPC 治疗的一种有前景的策略。
