猪氨肽酶N与F4 +产肠毒素大肠杆菌菌毛结合。
Porcine aminopeptidase N binds to F4+ enterotoxigenic Escherichia coli fimbriae.
作者信息
Xia Pengpeng, Wang Yiting, Zhu Congrui, Zou Yajie, Yang Ying, Liu Wei, Hardwidge Philip R, Zhu Guoqiang
机构信息
College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
出版信息
Vet Res. 2016 Feb 9;47:24. doi: 10.1186/s13567-016-0313-5.
F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+) E. coli to intestinal epithelial cells.
F4(+)产肠毒素大肠杆菌（ETEC）菌株可导致新生仔猪和断奶后仔猪腹泻病。已描述了几种不同的F4菌毛宿主受体，最近报道的是猪氨肽酶N（APN）。FaeG亚基对于三种F4变体与宿主细胞的结合至关重要。在这里，我们通过酵母双杂交和下拉试验表明，APN直接与来自三种血清型F4(+) ETEC的F4菌毛主要亚基FaeG结合。调节IPEC-J2细胞中APN基因的表达会影响ETEC的黏附。针对APN或F4菌毛产生的抗体均降低了ETEC的黏附。因此，APN介导F4(+)大肠杆菌与肠上皮细胞的附着。
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