Sufiandi Sandi, Obara Hiromichi, Enosawa Shin, Hsu Huai-Che, Matsuno Naoto, Mizunuma Hiroshi
Department of Mechanical Engineering, Graduate School of Science and Engineering, Tokyo Metropolitan University , Hachioji, Tokyo , Japan.
Department of Mechanical Engineering, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo, Japan; †Clinical Research Center, National Center for Child Health and Development, Setagaya-ku, Tokyo, Japan.
Cell Med. 2014 Dec 12;7(2):59-66. doi: 10.3727/215517914X685150. eCollection 2015 Feb 8.
Improving cell viability and function are important for enhancing the clinical results of cell transplantation. The relationship between cell viability and shear stress remains unexplained, and sedimentation effects during the infusion process are important to the hepatocyte transplantation process. In the present study, the relationship between cell viability and shear stress in the presence of sedimentation effect was investigated using a microchannel simulating the cell transplantation process under several shear stress conditions. Horizontal and vertical syringe orientations were employed to investigate the sedimentation effect. The vertical syringe orientation resulted in lower viability loss than the horizontal orientation. In summary, removing a sedimentation effect is important to improving cell viability by preventing high shear stress.
提高细胞活力和功能对于增强细胞移植的临床效果至关重要。细胞活力与剪切应力之间的关系尚不清楚，并且输注过程中的沉降效应对于肝细胞移植过程很重要。在本研究中，使用微通道模拟细胞移植过程，在几种剪切应力条件下研究了存在沉降效应时细胞活力与剪切应力之间的关系。采用水平和垂直注射器方向来研究沉降效应。垂直注射器方向导致的活力损失低于水平方向。总之，消除沉降效应对于通过防止高剪切应力来提高细胞活力很重要。
