MC3T3-E1细胞中纽蛋白表达对机械刺激的响应。

Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus.

作者信息

Cora-Cruz J J, Diffoot-Carlo N, Sundaram P A

机构信息

Department of Mechanical Engineering, University of Puerto Rico: Mayagüez Campus, Mayagüez, PR 00680, USA.

Department of Biology, University of Puerto Rico: Mayagüez Campus, Mayagüez, PR 00680, USA.

出版信息

Data Brief. 2015 Dec 5;6:94-100. doi: 10.1016/j.dib.2015.11.052. eCollection 2016 Mar.

DOI:10.1016/j.dib.2015.11.052

PMID:26858974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4706558/

Abstract

Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus.

摘要

已知加载频率会影响贴壁细胞粘着斑的表达。通过不同刚度的聚二甲基硅氧烷（PDMS）基底将小的周期性拉伸力传递给小鼠前成骨细胞MC3T3-E1细胞。通过纽蛋白的免疫荧光标记定量细胞在增殖和粘着斑特征方面的行为变化。与静态基底的结果相比，在受到循环刺激的基底上，无活性纽蛋白的量更高，而粘着斑的数量和面积则减少。无活性纽蛋白在细胞核附近的细胞质中呈云雾状出现。