Kaur Surleen, Anjali G, Bhardwaj Priya, Taneja Jyoti, Singh Rita
Division of Molecular Endocrinology and Reproduction, Department of Zoology, University of Delhi, Delhi 110007, India.
Data Brief. 2015 Dec 13;6:162-7. doi: 10.1016/j.dib.2015.12.001. eCollection 2016 Mar.
Insulin receptor substrate-2 (IRS-2) plays critical role in the regulation of various metabolic processes by insulin and IGF-1. The defects in its expression and/or function are linked to diseases like polycystic ovary syndrome (PCOS), insulin resistance and cancer. To predict the transcription factors (TFs) responsible for the regulation of human IRS-2 gene expression, the transcription factor binding sites (TFBS) and the corresponding TFs were investigated by analysis of IRS-2 promoter sequence using MatInspector Genomatix software (Cartharius et al., 2005 [1]). The ibid data is part of author׳s publication (Anjali et al., 2015 [2]) that explains Follicle stimulating hormone (FSH) mediated IRS-2 promoter activation in human granulosa cells and its importance in the pathophysiology of PCOS. Further analysis was carried out for binary interactions of TF regulatory genes in IRS-2 network using Cytoscape software tool and R-code. In this manuscript, we describe the methodology used for the identification of TFBSs in human IRS-2 promoter region and provide details on experimental procedures, analysis method, validation of data and also the raw files. The purpose of this article is to provide the data on all TFBSs in the promoter region of human IRS-2 gene as it has the potential for prediction of the regulation of IRS-2 gene in normal or diseased cells from patients with metabolic disorders and cancer.
胰岛素受体底物-2（IRS-2）在胰岛素和胰岛素样生长因子-1对各种代谢过程的调节中起关键作用。其表达和/或功能缺陷与多囊卵巢综合征（PCOS）、胰岛素抵抗和癌症等疾病有关。为了预测负责调控人类IRS-2基因表达的转录因子（TFs），使用MatInspector Genomatix软件（Cartharius等人，2005 [1]）分析IRS-2启动子序列，研究转录因子结合位点（TFBS）和相应的转录因子。上述数据是作者发表文章（Anjali等人，2015 [2]）的一部分，该文章解释了促卵泡激素（FSH）介导的人类颗粒细胞中IRS-2启动子激活及其在PCOS病理生理学中的重要性。使用Cytoscape软件工具和R代码对IRS-2网络中TF调控基因的二元相互作用进行了进一步分析。在本手稿中，我们描述了用于鉴定人类IRS-2启动子区域中TFBS的方法，并提供了实验程序、分析方法、数据验证以及原始文件的详细信息。本文的目的是提供人类IRS-2基因启动子区域所有TFBS的数据，因为它有可能预测代谢紊乱和癌症患者正常或患病细胞中IRS-2基因的调控情况。
