Candreva Ángela María, Smaldini Paola Lorena, Curciarello Renata, Fossati Carlos Alberto, Docena Guillermo Horacio, Petruccelli Silvana
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, and ‡Instituto de Estudios Inmunológicos y Fisiopatológicos (IIFP)- CONICET, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata , La Plata, 1900, Argentina.
J Agric Food Chem. 2016 Feb 24;64(7):1590-9. doi: 10.1021/acs.jafc.5b05623. Epub 2016 Feb 9.
Reactions to soy have been reported in a proportion of patients with IgE-mediated cow's milk allergy (CMA). In this work, we analyzed if Gly m Bd 28K/P28, one of the major soybean allergens, is a cross-reactive allergen with cow milk proteins (CMP). We showed that P28 was recognized by IgE sera from CMA patients and activated human peripheral basophils degranulation. Moreover, IgE sera of mice exclusively sensitized to CMP recognized P28. Splenocytes from sensitized animals secreted IL-5 and IL-13 when incubated with CMP or soy proteins, but only IL-13 when treated with P28. In addition, a skin test was strongly positive for CMP and weakly positive for P28. Remarkably, milk-sensitized mice showed hypersensitivity symptoms following sublingual challenge with P28 or CMP. With the use of bioinformatics' tools seven putative cross-reactive epitopes were identified. In conclusion, using in vitro and in vivo tests we demonstrated that P28 is a novel cross-reactive allergen with CMP.
据报道，一部分患有IgE介导的牛奶过敏（CMA）的患者会对大豆产生反应。在这项研究中，我们分析了大豆主要过敏原之一Gly m Bd 28K/P28是否是与牛奶蛋白（CMP）交叉反应的过敏原。我们发现P28能被CMA患者的IgE血清识别，并激活人外周血嗜碱性粒细胞脱颗粒。此外，仅对CMP致敏的小鼠的IgE血清也能识别P28。致敏动物的脾细胞在与CMP或大豆蛋白孵育时会分泌IL-5和IL-13，但在用P28处理时仅分泌IL-13。此外，皮肤试验对CMP呈强阳性，对P28呈弱阳性。值得注意的是，牛奶致敏的小鼠在经P28或CMP舌下激发后出现过敏症状。利用生物信息学工具鉴定出了7个假定的交叉反应表位。总之，通过体外和体内试验，我们证明P28是一种与CMP新的交叉反应过敏原。
