Wilson D B, Wyatt D P
Department of Surgery, School of Medicine, University of California, San Diego, La Jolla 92093.
Acta Neuropathol. 1989;79(1):94-100. doi: 10.1007/BF00308963.
Ultrastructural pathology in the apical neural folds was analyzed by means of tannic acid (TA) and ruthenium red (RR) cytochemistry in abnormal (vl/vl) mutant mouse embryos ranging in age from 17-35 somites. At lumbosacral levels of the spinal cord where closure fails to occur, as well as at more cranial levels where closure occurs but results in dorsal midline abnormalities, normal deposition of TA-positive and RR-positive material occurred in the space that develops between the overlying surface ectoderm (SE) and neuroepithelium (NE). However, in lumbosacral regions, pleomorphic excrescences projected abnormally from the apices of the transitional zone cells between SE and NE cells of the open neural folds. These abnormal projections consisted of enlarged cytoplasmic blebs, as well as entire cells. The cells were not necrotic nor did they show evidence of incipient degeneration. However, it is possible that they represent aberrant putative neural crest cells, as indicated by their location in the transitional zone and by the filopodia and lamellipodia projecting from their luminal surfaces.
通过单宁酸（TA）和钌红（RR）细胞化学方法，对17至35体节龄的异常（vl/vl）突变小鼠胚胎的顶神经褶超微结构病理学进行了分析。在脊髓腰骶部水平，此处闭合未能发生，以及在更靠头端的水平，此处虽发生闭合但导致背侧中线异常，在覆盖的表面外胚层（SE）和神经上皮（NE）之间发育的间隙中，TA阳性和RR阳性物质正常沉积。然而，在腰骶部区域，多形性赘生物从开放神经褶的SE和NE细胞之间的过渡区细胞顶端异常突出。这些异常突起由扩大的细胞质泡以及完整细胞组成。这些细胞并非坏死，也未显示出早期退化的迹象。然而，鉴于它们位于过渡区以及从其管腔表面伸出的丝状伪足和片状伪足，有可能它们代表异常的假定神经嵴细胞。
