Rainey P M
Department of Laboratory Medicine, Yale School of Medicine, New Haven, Connecticut 06510.
Am J Clin Pathol. 1989 Dec;92(6):779-86. doi: 10.1093/ajcp/92.6.779.
Digoxin immune Fab (ovine) (Digibind) is a preparation of Fab fragments of antidigoxin antibodies that is used as an antidote for severe digitalis toxicity. To assess the effects of its presence on digoxin immunoassays, specimens were prepared that simulated typical samples from treated patients and were distributed to a number of laboratories for analysis as unknowns. The antidote interfered to a varying extent with all assays reported. The results suggest that most competitive immunoassays will yield results that bear little relation to concentrations of total digoxin or free digoxin (i.e., digoxin not bound to the antidote). The aca, Dimension, and CEDIA sequential immunoassays moderately overestimated the free digoxin concentration. Free digoxin levels appeared to be only slightly overestimated by the Aria, Stratus, and Syva assays. The TDx Digoxin II assay yielded slight to moderate underestimates of the total digoxin concentration.
地高辛免疫Fab（羊源）（Digibind）是抗地高辛抗体的Fab片段制剂，用作严重洋地黄中毒的解毒剂。为评估其存在对地高辛免疫测定的影响，制备了模拟治疗患者典型样本的标本，并作为未知样本分发给多个实验室进行分析。该解毒剂对所有报告的测定均有不同程度的干扰。结果表明，大多数竞争性免疫测定得出的结果与总地高辛或游离地高辛（即未与解毒剂结合的地高辛）浓度几乎无关。aca、Dimension和CEDIA连续免疫测定适度高估了游离地高辛浓度。Aria、Stratus和Syva测定似乎仅略微高估了游离地高辛水平。TDx地高辛II测定对总地高辛浓度有轻微至中度的低估。
