Chisanga Chrispin, Eggert Dawn, Mitchell Charles D, Wood Charles, Angeletti Peter C
Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.
University of Miami Miller School of Medicine, Miami, FL, USA.
J Cancer Ther. 2015 Dec;6(15):1276-1289. doi: 10.4236/jct.2015.615140. Epub 2015 Dec 25.
Human papillomaviruses (HPVs) have previously been reported to infect epithelial trophoblast cells of the placenta. To investigate this possibility, 200 placental samples from Zambian women were separated into HIV+ and HIV- groups and tested for HPV by redundant primer PCR, using GP5+/GP6+ and CPI/CPII primer sets. Three HPV genotypes (HPV6, 16 and 90) were detected in placental samples. Whereas, 20 different HPV genotypes were detected in vaginal sampling of the same patients, suggesting that compartment specific sub-populations of HPV may exist. The incidence of HPV16 in placental samples was almost 2-fold greater in HIV+ women compared to HIV- (p = 0.0241). HPV16 L1 expression, detected by immunochemistry, was significantly higher in HIV+ than HIV- samples (p = 0.0231). HPV16 DNA was detected in the nuclei of trophoblast cells by hybridization. Overall, these results suggest that HPVs infect the placenta and that HIV significantly influences these infections.
此前有报道称人乳头瘤病毒（HPV）可感染胎盘的上皮滋养层细胞。为研究这种可能性，将200份赞比亚女性的胎盘样本分为HIV阳性和HIV阴性两组，使用GP5+/GP6+和CPI/CPII引物组，通过冗余引物PCR对HPV进行检测。在胎盘样本中检测到三种HPV基因型（HPV6、16和90）。然而，在同一患者的阴道样本中检测到20种不同的HPV基因型，这表明HPV可能存在特定部位的亚群。与HIV阴性女性相比，HIV阳性女性胎盘样本中HPV16的发生率几乎高出2倍（p = 0.0241）。通过免疫化学检测发现，HIV阳性样本中HPV16 L1的表达明显高于HIV阴性样本（p = 0.0231）。通过杂交在滋养层细胞核中检测到HPV16 DNA。总体而言，这些结果表明HPV可感染胎盘，且HIV对这些感染有显著影响。
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