Mauleekoonphairoj John, Puenpa Jiratchaya, Korkong Sumeth, Vongpunsawad Sompong, Poovorawan Yong
Southeast Asian J Trop Med Public Health. 2015 Nov;46(6):1013-20.
Human enterovirus (EV) infection causes hand, foot, and mouth disease (HFMD) and herpangina (HA). We studied the prevalence of enterovirus (EV) among patients with HFMD and HA in Thailand during 2013. We conducted a study in archived specimens of patients sent for screening for enterovirus. A total of 203 clinical specimens from 184 individuals with painful blister in the oropharynx and on the palms, soles, knees, elbows or buttock were examined by semi-nested polymerase chain reaction (PCR) for the 5'UTR and VP1 genes of EV. Eighty-six samples were positive: EV71 was detected in 14 (30%), CV-A8 in 12 (26%) and CV-A16 in 10 (21%). Classification of EV species detected revealed that 46 specimens were EV-A, 14 specimens were EV-B, 1 specimen was EV-D, and 16 specimens were positive for unclassified enterovirus. The majority of individuals with EV infection were aged 2-6 years. Multiple EV-A serotypes were detected among HFMD and HA patients in our study.
人肠道病毒（EV）感染可引发手足口病（HFMD）和疱疹性咽峡炎（HA）。我们研究了2013年泰国手足口病和疱疹性咽峡炎患者中肠道病毒（EV）的流行情况。我们对送检进行肠道病毒筛查的患者存档标本开展了一项研究。通过半巢式聚合酶链反应（PCR）检测了来自184名口咽、手掌、脚底、膝盖、肘部或臀部出现疼痛性水疱的个体的总共203份临床标本中肠道病毒的5'UTR和VP1基因。86份样本呈阳性：14份（30%）检测到肠道病毒71型（EV71），12份（26%）检测到柯萨奇病毒A8型（CV-A8），10份（21%）检测到柯萨奇病毒A16型（CV-A16）。所检测到的肠道病毒种类分类显示，46份标本为A组肠道病毒（EV-A），14份标本为B组肠道病毒（EV-B），1份标本为D组肠道病毒（EV-D），16份标本为未分类肠道病毒阳性。大多数肠道病毒感染个体年龄在2至6岁。在我们的研究中，手足口病和疱疹性咽峡炎患者中检测到多种A组肠道病毒血清型。
