• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于预测结肠腺癌的分子靶点鉴定

Identification of Molecular Targets for Predicting Colon Adenocarcinoma.

作者信息

Wang Yansheng, Zhang Jun, Li Li, Xu Xin, Zhang Yong, Teng Zhaowei, Wu Feihu

机构信息

Department of General Surgery, The People's Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunan, China (mainland).

Department of Orthopedic Surgery, The People's Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunan, China (mainland).

出版信息

Med Sci Monit. 2016 Feb 12;22:460-8. doi: 10.12659/msm.895881.

DOI:10.12659/msm.895881
PMID:26868022
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4754092/
Abstract

BACKGROUND Colon adenocarcinoma mostly happens at the junction of the rectum and is a common gastrointestinal malignancy. Accumulated evidence has indicated that colon adenocarcinoma develops by genetic alterations and is a complicated disease. The aim of this study was to screen differentially expressed miRNAs (DEMs) and genes with diagnostic and prognostic potentials in colon adenocarcinoma. MATERIAL AND METHODS In this study we screened DEMs and their target genes (DEGs) between 100 colon adenocarcinoma and normal samples in The Cancer Genome Atlas (TCGA) database by using the DEseq toolkit in Bioconductor. Then Go enrichment and KEGG pathway analysis were performed on the selected differential genes by use of the DAVID online tool. A regulation network of miRNA-gene was constructed and analyzed by Cytoscape. Finally, we performed ROC analysis of 8 miRNAs and ROC curves were drawn. RESULTS A total of 159 DEMs and 1921 DEGs were screened, and 1881 pairs of miRNA-target genes with significant negative correlations were also obtained. A regulatory network of miRNA-gene, including 60 cancer-related genes and 47 miRNAs, was successfully constructed. In addition, 5 clusters with several miRNAs regulating a set of target genes simultaneously were identified through cluster analysis. There were 8 miRNAs involved in these 5 clusters, and these miRNAs could serve as molecular biomarkers to distinguish colon adenocarcinoma and normal samples indicated by ROC analysis. CONCLUSIONS The identified 8 miRNAs were closely associated with colon adenocarcinoma, which may have great clinical value as diagnostic and prognostic biomarkers and provide new ideas for targeted therapy.

摘要

背景

结肠腺癌多发生于直肠交界处，是一种常见的胃肠道恶性肿瘤。越来越多的证据表明，结肠腺癌是由基因改变发展而来的复杂疾病。本研究旨在筛选在结肠腺癌中具有诊断和预后潜力的差异表达微小RNA（DEM）和基因。

材料与方法

在本研究中，我们使用生物导体中的DEseq工具包，在癌症基因组图谱（TCGA）数据库中的100个结肠腺癌样本和正常样本之间筛选DEM及其靶基因（DEG）。然后利用DAVID在线工具对选定的差异基因进行基因本体（Go）富集分析和京都基因与基因组百科全书（KEGG）通路分析。通过Cytoscape构建并分析miRNA-基因调控网络。最后，我们对8个miRNA进行了ROC分析并绘制了ROC曲线。

结果

共筛选出159个DEM和1921个DEG，还获得了1881对具有显著负相关的miRNA-靶基因。成功构建了一个包含60个癌症相关基因和47个miRNA的miRNA-基因调控网络。此外，通过聚类分析鉴定出5个簇，其中几个miRNA同时调控一组靶基因。这5个簇中有8个miRNA，ROC分析表明这些miRNA可作为区分结肠腺癌和正常样本的分子生物标志物。

结论

所鉴定的8个miRNA与结肠腺癌密切相关，可能作为诊断和预后生物标志物具有重要临床价值，并为靶向治疗提供新思路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/1e4384a12c37/medscimonit-22-460-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/7b07cb0f99be/medscimonit-22-460-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/6c8bf4df6cea/medscimonit-22-460-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/9b5409c48862/medscimonit-22-460-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/666799f67362/medscimonit-22-460-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/fc5868b07786/medscimonit-22-460-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/1e4384a12c37/medscimonit-22-460-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/7b07cb0f99be/medscimonit-22-460-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/6c8bf4df6cea/medscimonit-22-460-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/9b5409c48862/medscimonit-22-460-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/666799f67362/medscimonit-22-460-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/fc5868b07786/medscimonit-22-460-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a82/4754092/1e4384a12c37/medscimonit-22-460-g006.jpg

相似文献

1
Identification of Molecular Targets for Predicting Colon Adenocarcinoma.用于预测结肠腺癌的分子靶点鉴定
Med Sci Monit. 2016 Feb 12;22:460-8. doi: 10.12659/msm.895881.
2
Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing.通过RNA测序筛选早期结肠腺癌中的关键基因和微小RNA
Tumour Biol. 2017 Jul;39(7):1010428317714899. doi: 10.1177/1010428317714899.
3
Identification of hub genes and construction of transcriptional regulatory network for the progression of colon adenocarcinoma hub genes and TF regulatory network of colon adenocarcinoma.结直肠癌进展的枢纽基因鉴定及转录调控网络构建。
J Cell Physiol. 2020 Mar;235(3):2037-2048. doi: 10.1002/jcp.29067. Epub 2019 Oct 14.
4
Identification of prognostic miRNA biomarkers for predicting overall survival of colon adenocarcinoma and bioinformatics analysis: A study based on The Cancer Genome Atlas database.用于预测结肠腺癌总生存期的预后性miRNA生物标志物的鉴定及生物信息学分析：一项基于癌症基因组图谱数据库的研究
J Cell Biochem. 2019 Jun;120(6):9839-9849. doi: 10.1002/jcb.28264. Epub 2018 Dec 9.
5
A microRNA expression signature as a predictor of survival for colon adenocarcinoma.一种 microRNA 表达特征作为结直肠腺癌患者生存预测的标志物。
Neoplasma. 2017;64(1):56-64. doi: 10.4149/neo_2017_107.
6
Screening of Target Genes and Regulatory Function of miRNAs as Prognostic Indicators for Prostate Cancer.作为前列腺癌预后指标的靶基因筛选及miRNA的调控功能
Med Sci Monit. 2015 Dec 2;21:3748-59. doi: 10.12659/msm.894670.
7
Identification of molecular targets for esophageal carcinoma diagnosis using miRNA-seq and RNA-seq data from The Cancer Genome Atlas: a study of 187 cases.利用来自癌症基因组图谱的miRNA测序和RNA测序数据鉴定食管癌诊断的分子靶点：一项187例病例的研究
Oncotarget. 2017 May 30;8(22):35681-35699. doi: 10.18632/oncotarget.16051.
8
Comprehensive analysis of microRNA/mRNA signature in colon adenocarcinoma.结直肠腺癌中 microRNA/mRNA 特征的综合分析。
Eur Rev Med Pharmacol Sci. 2017 May;21(9):2114-2129.
9
Bioinformatics analysis of RNA-seq data revealed critical genes in colon adenocarcinoma.RNA-seq 数据的生物信息学分析揭示了结肠癌中的关键基因。
Eur Rev Med Pharmacol Sci. 2017 Jul;21(13):3012-3020.
10
Genome‑scale analysis to identify potential prognostic microRNA biomarkers for predicting overall survival in patients with colon adenocarcinoma.基于基因组规模的分析鉴定预测结肠腺癌患者总生存期的潜在预后 microRNA 生物标志物。
Oncol Rep. 2018 Oct;40(4):1947-1958. doi: 10.3892/or.2018.6607. Epub 2018 Jul 30.

引用本文的文献

1
SETBP1 activation upon MDM4-enhanced ubiquitination of NR3C1 triggers dissemination of colorectal cancer cells.SETBP1 的激活是由于 MDM4 增强了 NR3C1 的泛素化，从而触发了结直肠癌细胞的扩散。
Clin Exp Metastasis. 2024 Oct;41(5):747-764. doi: 10.1007/s10585-024-10294-2. Epub 2024 May 26.
2
Molecular functions of microRNAs in colorectal cancer: recent roles in proliferation, angiogenesis, apoptosis, and chemoresistance.微小 RNA 在结直肠癌中的分子功能：在增殖、血管生成、凋亡和化疗耐药性中的最新作用。
Naunyn Schmiedebergs Arch Pharmacol. 2024 Aug;397(8):5617-5630. doi: 10.1007/s00210-024-03076-w. Epub 2024 Apr 15.
3

本文引用的文献

1
DeDaL: Cytoscape 3 app for producing and morphing data-driven and structure-driven network layouts.DeDaL：用于生成和变形数据驱动及结构驱动网络布局的Cytoscape 3应用程序。
BMC Syst Biol. 2015 Aug 14;9:46. doi: 10.1186/s12918-015-0189-4.
2
Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma.结直肠癌中癌症相关基因及其调控性微小RNA的差异表达
Gene. 2015 Aug 1;567(1):81-6. doi: 10.1016/j.gene.2015.04.065. Epub 2015 Apr 27.
3
Progesterone receptor status in determining the prognosis of estrogen receptor positive/ HER2 negative breast carcinoma patients.
Multimerin-1 and cancer: a review.
多聚蛋白-1 与癌症：综述。
Biosci Rep. 2022 Feb 25;42(2). doi: 10.1042/BSR20211248.
4
lncRNA-SNHG17 promotes colon adenocarcinoma progression and serves as a sponge for miR-375 to regulate CBX3 expression.长链非编码RNA-SNHG17促进结肠腺癌进展，并作为miR-375的海绵以调控CBX3表达。
Am J Transl Res. 2020 Sep 15;12(9):5283-5295. eCollection 2020.
5
Analysis of CASP12 diagnostic and prognostic values in cervical cancer based on TCGA database.基于 TCGA 数据库分析 CASP12 在宫颈癌中的诊断和预后价值。
Biosci Rep. 2019 Dec 20;39(12). doi: 10.1042/BSR20192706.
6
Differentially expressed gene profile and relevant pathways of the traditional Chinese medicine cinobufotalin on MCF‑7 breast cancer cells.中药华蟾素对 MCF-7 乳腺癌细胞差异表达基因谱及相关通路的研究。
Mol Med Rep. 2019 May;19(5):4256-4270. doi: 10.3892/mmr.2019.10062. Epub 2019 Mar 19.
7
shRNA-induced silencing of Ras-related C3 botulinum toxin substrate 1 inhibits the proliferation of colon cancer cells through upregulation of BAD and downregulation of cyclin D1.shRNA 诱导的 Ras 相关 C3 型肉毒杆菌毒素底物 1 沉默通过上调 BAD 和下调细胞周期蛋白 D1 抑制结肠癌细胞的增殖。
Int J Mol Med. 2018 Mar;41(3):1397-1408. doi: 10.3892/ijmm.2017.3345. Epub 2017 Dec 22.
8
Expression analysis of microRNA as prognostic biomarkers in colorectal cancer.作为结直肠癌预后生物标志物的微小RNA表达分析
Oncotarget. 2016 Dec 26;8(32):52403-52412. doi: 10.18632/oncotarget.14175. eCollection 2017 Aug 8.
9
CytoCluster: A Cytoscape Plugin for Cluster Analysis and Visualization of Biological Networks.CytoCluster：一款用于生物网络聚类分析和可视化的Cytoscape插件。
Int J Mol Sci. 2017 Aug 31;18(9):1880. doi: 10.3390/ijms18091880.
孕激素受体状态在确定雌激素受体阳性/人表皮生长因子受体2阴性乳腺癌患者预后中的作用
J BUON. 2015 Jan-Feb;20(1):28-34.
4
Novel role of Sarco/endoplasmic reticulum calcium ATPase 2 in development of colorectal cancer and its regulation by F36, a curcumin analog.肌浆网/内质网钙ATP酶2在结直肠癌发生发展中的新作用及其受姜黄素类似物F36的调控
Biomed Pharmacother. 2014 Oct;68(8):1141-8. doi: 10.1016/j.biopha.2014.10.014. Epub 2014 Oct 29.
5
Importance of metastatic lymph node ratio in non-metastatic, lymph node-invaded colon cancer: a clinical trial.非转移性、淋巴结受累结肠癌中转移淋巴结比率的重要性：一项临床试验
Med Sci Monit. 2014 Aug 4;20:1369-75. doi: 10.12659/MSM.890804.
6
Postmenopausal hormone receptor-positive advanced breast cancer.绝经后激素受体阳性晚期乳腺癌
Oncology (Williston Park). 2013 Jun;27(6):571-2, 574, 576 passim.
7
Insulin caused drug resistance to oxaliplatin in colon cancer cell line HT29.胰岛素导致结肠癌 HT29 细胞株对奥沙利铂产生耐药性。
J Gastrointest Oncol. 2011 Mar;2(1):27-33. doi: 10.3978/j.issn.2078-6891.2010.028.
8
The IntAct molecular interaction database in 2012.IntAct 分子相互作用数据库，2012 年版。
Nucleic Acids Res. 2012 Jan;40(Database issue):D841-6. doi: 10.1093/nar/gkr1088. Epub 2011 Nov 24.
9
miR-21 targets the tumor suppressor RhoB and regulates proliferation, invasion and apoptosis in colorectal cancer cells.miR-21 靶向肿瘤抑制因子 RhoB，调节结直肠癌细胞的增殖、侵袭和凋亡。
FEBS Lett. 2011 Oct 3;585(19):2998-3005. doi: 10.1016/j.febslet.2011.08.014. Epub 2011 Aug 26.
10
MicroRNA-21 exhibits antiangiogenic function by targeting RhoB expression in endothelial cells.MicroRNA-21 通过靶向内皮细胞中 RhoB 的表达发挥抗血管生成功能。
PLoS One. 2011 Feb 10;6(2):e16979. doi: 10.1371/journal.pone.0016979.