Breidbach Andreas, Ulberth Franz
European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg 111, B-2440 Geel, Belgium.
Food Chem. 2016 Jun 15;201:52-8. doi: 10.1016/j.foodchem.2016.01.032. Epub 2016 Jan 9.
Irradiation of food products and ingredients must be indicated by proper labeling. This study evaluated the appropriateness of the European Standard EN 1785:2003 for the detection of 2-alkylcyclobutanones, which are radiolysis products of fatty acids, in cashew nuts and nutmeg and confirmed its suitability to detect irradiation of cashew nut samples at average absorbed doses of 1 kGy and above. An alternative method was developed, which is based on matrix solid phase dispersion and subsequent separation and detection of oxime derivatives of 2-alkylcyclobutanones by high performance-high resolution mass spectrometry. It is more rapid, less resource consuming, and more sensitive than EN 1785:2003. This method allowed detection of 2-alkylcyclobutanones in cashew nuts irradiated at 100 Gray and in nutmeg irradiated at 400 Gray. None of the 26 cashew nut and 14 nutmeg samples purchased in different EU Member States contained traces of 2-alkylcyclobutanones.
食品和食品配料的辐照必须通过适当的标签加以标明。本研究评估了欧洲标准EN 1785:2003用于检测腰果和肉豆蔻中脂肪酸辐解产物2-烷基环丁酮的适用性，并证实了其适用于检测平均吸收剂量为1千戈瑞及以上的腰果样品的辐照情况。开发了一种替代方法，该方法基于基质固相分散，随后通过高性能高分辨率质谱对2-烷基环丁酮的肟衍生物进行分离和检测。它比EN 1785:2003更快速、资源消耗更少且更灵敏。该方法能够检测出辐照剂量为100戈瑞的腰果以及辐照剂量为400戈瑞的肉豆蔻中的2-烷基环丁酮。在不同欧盟成员国购买的26个腰果样品和14个肉豆蔻样品中均未检测到2-烷基环丁酮的痕迹。
